EphA7 regulates claudin6 and pronephros development in Xenopus

Sun, Jian1,4; Wang, Xiaolei1; Shi, Yu1,3; Li, Jiejing1; Li, Chaocui1; Shi, Zhaoying2; Chen, Yonglong2; Mao, Bingyu1

2018-01-08

10.1016/j.bbrc.2017.12.027

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   影响因子和分区

0006-291X

1090-2104

Eph/ephrin molecules are widely expressed during embryonic development, and function in a variety of developmental processes. Here we studied the roles of the Eph receptor EphA7 and its soluble form in Xenopus pronephros development. EphA7 is specifically expressed in pronephric tubules at tadpole stages and knockdown of EphA7 by a translation blocking morpholino led to defects in tubule cell differentiation and morphogenesis. A soluble form of EphA7 (sEphA7) was also identified. Interestingly, the membrane level of claudin6 (CLDN6), a tetraspan transmembrane tight junction protein, was dramatically reduced in the translation blocking morpholino injected embryos, but not when a splicing morpholino was used, which blocks only the full length EphA7. In cultured cells, EphA7 binds and phosphorylates CLDN6, and reduces its distribution at the cell surface. Our work suggests a role of EphA7 in the regulation of cell adhesion during pronephros development, whereas sEphA7 works as an antagonist. (C) 2017 Elsevier Inc. All rights reserved.

EphA7 Soluble EphA7 Pronephros development Morphogenesis Xenopus Claudin6

SCI

英语

其他

Shenzhen Key Laboratory of Cell Microenvironment[ZDSYS20140509142721429]

Biochemistry & Molecular Biology ; Biophysics

Biochemistry & Molecular Biology ; Biophysics

WOS:000424732700002

ACADEMIC PRESS INC ELSEVIER SCIENCE

BIOLOGY & BIOCHEMISTRY

Web of Science

1.Chinese Acad Sci, Kunming Inst Zool, State Key Lab Genet Resources & Evolut, Kunming, Yunnan, Peoples R China
2.Southern Univ Sci & Technol, Dept Biol, Shenzhen Key Lab Cell Microenvironm, Shenzhen, Guangdong, Peoples R China
3.Chongqing Med Univ, Childrens Hosp, Dept Clin Lab, Chongqing, Peoples R China
4.NCI, Canc & Dev Biol Lab, NIH, Frederick, MD 21702 USA

Sun, Jian,Wang, Xiaolei,Shi, Yu,et al. EphA7 regulates claudin6 and pronephros development in Xenopus[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2018,495(2):1580-1587.

Sun, Jian.,Wang, Xiaolei.,Shi, Yu.,Li, Jiejing.,Li, Chaocui.,...&Mao, Bingyu.(2018).EphA7 regulates claudin6 and pronephros development in Xenopus.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,495(2),1580-1587.

Sun, Jian,et al."EphA7 regulates claudin6 and pronephros development in Xenopus".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 495.2(2018):1580-1587.