Imaging Fast Cellular Uptake of Polymer Dots via Receptor-Mediated Endocytosis

Sun, Zezhou1; Yuan, Ye1; Li, Qiong3; Liu, Zhihe1; Wu, Changfeng2

2018-01

10.1007/s41664-018-0048-6

Journal of Analysis and Testing   影响因子和分区

2096-241X

2509-4696

During the past decade, semiconducting polymer dots (Pdots) have been prevailing in the family of fluorescent probes due to its high photon budget, excellent photo stability, and good biocompatibility. In this study, holo-Transferrin human (Tf) was utilized to covalently couple with Pdots for a highly efficient endocytosis process through transferrin receptors (TfRs) mediated internalization. As a result, the endocytosis efficiency of Tf-conjugated Pdots in HeLa cells dramatically increased as compared to that of unconjugated Pdots in the same condition. This acute increment demonstrates that holo-Transferrin molecules are of great capability for intracellular delivery of Pdots to TfRs overexpressed cells. The transportation route of Tf-conjugated Pdots is quite different from the uptake mechanism of unconjugated Pdots via nonspecific endocytic trafficking pathway. Considering the overexpression of TfRs in various cancer cells, Tf-conjugated Pdots hold potential to function as a nanocarrier for efficient drug delivery in cancer diagnostics and therapy.

Conjugated polymer dot Transferrin receptors Fluorescence microscopy Holo-Transferrin

ESCI ; EI

英语

通讯

National Natural Science Foundation of China[61335001][81771930] ; Shenzhen Science and Technology Innovation Commission[JCYJ20170307110157501]

Chemistry

Chemistry, Analytical

WOS:000431438300006

SPRINGER SINGAPORE PTE LTD

20214311067209

Biocompatibility ; Budget control ; Controlled drug delivery ; Diseases ; Fluorescence ; Fluorescence microscopy ; Lanthanum compounds ; Molecular biology ; Targeted drug delivery ; Transportation routes

Biology:461.9 ; Immunology:461.9.1 ; Light/Optics:741.1 ; Organic Polymers:815.1.1 ; Quantum Theory; Quantum Mechanics:931.4

Web of Science

1.Jilin Univ, Coll Elect Sci & Engn, State Key Lab Integrated Optoelect, Changchun 130012, Jilin, Peoples R China;
2.Southern Univ Sci & Technol, Dept Biomed Engn, Shenzhen 518055, Peoples R China;
3.Linyi Univ, Shandong Prov Key Lab Detect Technol Tumor Marker, Coll Chem & Chem Engn, Linyi 276000, Shandong, Peoples R China

Sun, Zezhou,Yuan, Ye,Li, Qiong,et al. Imaging Fast Cellular Uptake of Polymer Dots via Receptor-Mediated Endocytosis[J]. Journal of Analysis and Testing,2018,2(1):61-68.

Sun, Zezhou,Yuan, Ye,Li, Qiong,Liu, Zhihe,&Wu, Changfeng.(2018).Imaging Fast Cellular Uptake of Polymer Dots via Receptor-Mediated Endocytosis.Journal of Analysis and Testing,2(1),61-68.

Sun, Zezhou,et al."Imaging Fast Cellular Uptake of Polymer Dots via Receptor-Mediated Endocytosis".Journal of Analysis and Testing 2.1(2018):61-68.