Conformational Hotspots of Dengue Virus NS5 RdRp

Introduction: Dengue virus is among the most widespread mosquito-borne human pathogens with 5 different serotypes. A ratio of 3 to 7 structural and non-structural proteins is retained by the 10.7 kb viral RNA genome. The Dengue virus NS5, a non-structural and most conserved protein in the genome plays vital role in virus replication machinery. The C-terminal RNA-dependent RNA polymerase (RdRp) domain of NS5 has been solved experimentally in the canonical right handed conformation that comprises of 3 sub-domains namely finger, palm and thumb. The presence of different structural characteristics portray that RdRp adopts various conformation strategies to fulfill functional modes.