STAT3-Inducible Mouse ESCs: A Model to Study the Role of STAT3 in ESC Maintenance and Lineage Differentiation

Wong, Yu Qian1,2; Xu, Hongyan1,3; Wu, Qiang1,4; Liu, Xinyu2; Lufei, Chengchen2; Xu, Xiu Qin5; Fu, Xin-Yuan1,2,6

2018

10.1155/2018/8632950

Stem Cells International   影响因子和分区

1687-966X

1687-9678

Studies have demonstrated that STAT3 is essential in maintaining self-renewal of embryonic stem cells (ESCs) and modulates ESC differentiation. However, there is still lack of direct evidence on STAT3 functions in ESCs and embryogenesis because constitutive STAT3 knockout (KO) mouse is embryonic lethal at E6.5-E7.5, prior to potential functional role in early development can be assessed. Therefore, in this study, two inducible STAT3 ESC lines were established, including the STAT3 knockout (InSTAT3 KO) and pSTAT3 overexpressed (InSTAT3 CA) using Tet-on inducible system in which STAT3 expression can be strictly controlled by doxycycline (Dox) stimulation. Through genotyping, deletion of STAT3 alleles was detected in InSTAT3 KO ESCs following 24 hours Dox stimulation. Western blot also showed that pSTAT3 and STAT3 protein levels were significantly reduced in InSTAT3 KO ESCs while dominantly elevated in InSTAT3 CA ECSs upon Dox stimulation. Likewise, it was found that STAT3-null ESCs would affect the differentiation of ESCs into mesoderm and cardiac lineage. Taken together, the findings of this study indicated that InSTAT3 KO and InSTAT3 CA ESCs could provide a new tool to clarify the direct targets of STAT3 and its role in ESC maintenance, which will facilitate the elaboration of the mechanisms whereby STAT3 maintains ESC pluripotency and regulates ESC differentiation during mammalian embryogenesis.

SCI

英语

通讯

National Natural Science Foundation of China (NSFC)[81670286]

Cell Biology

Cell & Tissue Engineering

WOS:000444890500001

HINDAWI LTD

Web of Science

1.Natl Univ Singapore, Yong Loo Lin Sch Med, Dept Biochem, 8 Med Dr, Singapore 117597, Singapore
2.Natl Univ Singapore, Canc Sci Inst Singapore, 14 Med Dr, Singapore 117599, Singapore
3.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Minist Agr, Key Lab Trop & Subtrop Fishery Resource Applicat, Guangzhou 510380, Guangdong, Peoples R China
4.Macau Univ Sci & Technol, State Key Lab Qual Res Chinese Med, Room 704a,Block H,Ave Wai Long, Taipa, Macau, Peoples R China
5.Xiamen Univ, Med Coll, Inst Stem Cell & Regenerat Med, Xiamen 361100, Fujian, Peoples R China
6.Southern Univ Sci & Technol, Dept Biol, 1088 Xueyuan Ave, Shenzhen Shi 518055, Guangdong Sheng, Peoples R China

Wong, Yu Qian,Xu, Hongyan,Wu, Qiang,et al. STAT3-Inducible Mouse ESCs: A Model to Study the Role of STAT3 in ESC Maintenance and Lineage Differentiation[J]. Stem Cells International,2018,2018.

Wong, Yu Qian.,Xu, Hongyan.,Wu, Qiang.,Liu, Xinyu.,Lufei, Chengchen.,...&Fu, Xin-Yuan.(2018).STAT3-Inducible Mouse ESCs: A Model to Study the Role of STAT3 in ESC Maintenance and Lineage Differentiation.Stem Cells International,2018.

Wong, Yu Qian,et al."STAT3-Inducible Mouse ESCs: A Model to Study the Role of STAT3 in ESC Maintenance and Lineage Differentiation".Stem Cells International 2018(2018).