A dual-specific IGF-I/II human engineered antibody domain inhibits IGF signaling in breast cancer cells

Chen, Zhizhen1; Liu, Jie1; Chu, Dafeng2; Shan, Yaming3; Ma, Guixing4; Zhang, Hongmin4; Zhang, Xiaohua Douglas1; Wang, Pu5; Chen, Qiang1; Deng, Chuxia1; Chen, Weizao6; Dimitrov, Dimiter S.7; Zhao, Qi1

2018

10.7150/ijbs.25928

International Journal of Biological Sciences   影响因子和分区

1449-2288

The insulin-like growth factors (IGFs), IGF-I and IGF-II, are essential for regulating cell growth, differentiation and metastasis of a broad range of malignancies. The IGF-I/II actions are mediated through the IGF receptor type 1 (IGF-1R) and the insulin receptor (IR), which are overexpressed in multiple types of tumors. Here, we have firstly identified a human engineered antibody domain (eAd) from a phage-displayed VH library. The eAd suppressed the signal transduction of IGF-1R mediated by exogenous IGF-I or IGF-II in breast cancer cell lines through neutralizing both IGF-I and IGF-II. It also significantly inhibited the growth of breast cancer cells. Therefore, the anti-IGF-I/II eAd offers an alternative approach to target both the IGF-1R signaling pathways through the inhibition of IGF-I/II.

Insulin-like growth factor Yeast display Affinity maturation eAd

SCI

英语

其他

[SRG2016-00082-FHS]

Biochemistry & Molecular Biology ; Life Sciences & Biomedicine - Other Topics

Biochemistry & Molecular Biology ; Biology

WOS:000433261800011

IVYSPRING INT PUBL

Web of Science

1.Univ Macau, Fac Hlth Sci, Macau, Peoples R China
2.Univ Calif Los Angeles, Dept Bioengn, Los Angeles, CA USA
3.Jilin Univ, Sch Life Sci, Natl Engn Lab AIDS Vaccine, Changchun, Jilin, Peoples R China
4.Southern Univ Sci & Technol, Dept Biol, Guangdong Prov Key Lab Cell Microenvironm & Dis R, Shenzhen Key Lab Cell Microenvironm,SUSTech HKU J, Shenzhen, Guangdong, Peoples R China
5.Chinese Acad Sci, Shenzhen Inst Adv Technol, Shenzhen, Guangdong, Peoples R China
6.NCI, Ctr Canc Res, NIH, Frederick, MD 21701 USA
7.Univ Pittsburgh, Med Sch, Ctr Antibody Therapeut, Pittsburgh, PA 15260 USA

Chen, Zhizhen,Liu, Jie,Chu, Dafeng,et al. A dual-specific IGF-I/II human engineered antibody domain inhibits IGF signaling in breast cancer cells[J]. International Journal of Biological Sciences,2018,14(7):799-806.

Chen, Zhizhen.,Liu, Jie.,Chu, Dafeng.,Shan, Yaming.,Ma, Guixing.,...&Zhao, Qi.(2018).A dual-specific IGF-I/II human engineered antibody domain inhibits IGF signaling in breast cancer cells.International Journal of Biological Sciences,14(7),799-806.

Chen, Zhizhen,et al."A dual-specific IGF-I/II human engineered antibody domain inhibits IGF signaling in breast cancer cells".International Journal of Biological Sciences 14.7(2018):799-806.