| 题名 | BL-Hi-C is an efficient and sensitive approach for capturing structural and regulatory chromatin interactions |
| 作者 | |
| 通讯作者 | Zhang, Michael Q.; Chen, Yang |
| 发表日期 | 2017-11-20
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| DOI | |
| 发表期刊 | |
| ISSN | 2041-1723
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| 卷号 | 8 |
| 摘要 | In human cells, DNA is hierarchically organized and assembled with histones and DNA-binding proteins in three dimensions. Chromatin interactions play important roles in genome architecture and gene regulation, including robustness in the developmental stages and flexibility during the cell cycle. Here we propose in situ Hi-C method named Bridge Linker-Hi-C (BL-Hi-C) for capturing structural and regulatory chromatin interactions by restriction enzyme targeting and two-step proximity ligation. This method improves the sensitivity and specificity of active chromatin loop detection and can reveal the regulatory enhancer-promoter architecture better than conventional methods at a lower sequencing depth and with a simpler protocol. We demonstrate its utility with two well-studied developmental loci: the beta-globin and HOXC cluster regions. |
| 相关链接 | [来源记录] |
| 收录类别 | |
| 语种 | 英语
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| 重要成果 | NI论文
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| 学校署名 | 其他
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| 资助项目 | Beijing Natural Science Foundation[7151009]
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| WOS研究方向 | Science & Technology - Other Topics
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| WOS类目 | Multidisciplinary Sciences
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| WOS记录号 | WOS:000415759900005
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| 出版者 | |
| 来源库 | Web of Science
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| 引用统计 |
被引频次[WOS]：55
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| 成果类型 | 期刊论文 |
| 条目标识符 | http://sustech.caswiz.com/handle/2SGJ60CL/28422 |
| 专题 | 前沿与交叉科学研究院 |
| 作者单位 | 1.Tsinghua Univ, MOE Key Lab Bioinformat, Beijing 100084, Peoples R China 2.Tsinghua Univ, TNLIST, Bioinformat Div, Beijing 100084, Peoples R China 3.Tsinghua Univ, Ctr Synthet & Syst Biol, TNLIST, Beijing 100084, Peoples R China 4.Tsinghua Univ, Sch Med, Beijing 100084, Peoples R China 5.Tsinghua Univ, MOE Key Lab Bioinformat, Beijing 100084, Peoples R China 6.Tsinghua Univ, TNLIST, Bioinformat Div, Beijing 100084, Peoples R China 7.Tsinghua Univ, TNLIST, Ctr Synthet & Syst Biol, Beijing 100084, Peoples R China 8.Tsinghua Univ, Dept Automat, Beijing 100084, Peoples R China 9.Southern Univ Sci & Technol, SUSTech Acad Adv Interdisciplinary Studies, Medi X Inst, Shenzhen 518055, Peoples R China 10.Peking Univ, Sch Math Sci, Beijing 100871, Peoples R China 11.Univ Texas Dallas, Dept Biol Sci, Ctr Syst Biol, 800 West Campbell Rd,RL11, Richardson, TX 75080 USA |
| 推荐引用方式 GB/T 7714 |
Liang, Zhengyu,Li, Guipeng,Wang, Zejun,et al. BL-Hi-C is an efficient and sensitive approach for capturing structural and regulatory chromatin interactions[J]. Nature Communications,2017,8.
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| APA |
Liang, Zhengyu.,Li, Guipeng.,Wang, Zejun.,Djekidel, Mohamed Nadhir.,Li, Yanjian.,...&Chen, Yang.(2017).BL-Hi-C is an efficient and sensitive approach for capturing structural and regulatory chromatin interactions.Nature Communications,8.
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| MLA |
Liang, Zhengyu,et al."BL-Hi-C is an efficient and sensitive approach for capturing structural and regulatory chromatin interactions".Nature Communications 8(2017).
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| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | 操作 | |
| s41467-017-01754-3.p（1720KB） | -- | -- | 开放获取 | -- | 浏览 | |
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