Multi-Omics Analyses Revealed GOLT1B as a Potential Prognostic Gene in Breast Cancer Probably Regulating the Immune Microenvironment

Liu, Junping1,2,3; Zhang, Wei1,2; Cai, Wanxia1; Chen, Yumei1; Cai, Xiaozhong4; Tang, Donge1; Tang, Min3; Dai, Yong1

2022-01-19

10.3389/fonc.2021.805273

Frontiers in Oncology   影响因子和分区

2234-943X

As recently reported by The International Agency for Research on Cancer (IARC), breast cancer has the highest incidence of all cancers in 2020. Many studies have revealed that golgi apparatus is closely associated with the development of breast cancer. However, the role of golgi apparatus in immune microenvironment is still not clear. In this study, using RNA-Seq datasets of breast cancer patients from the public database (n = 1080), we revealed that GOLT1B, encoding a golgi vesicle transporter protein, was significantly higher expressed in human breast cancer tissues versus normal tissues. Besides, we verified GOLT1B expression in five breast cancer cell line using our original data and found GOLT1B was significantly up-regulated in MDA-MB-231, MCF-7, SKBR3. Subsequently, we identified GOLT1B as a potential independent prognostic factor for breast cancer. After a multi-omics analysis, we uncovered that the higher expression of GOLT1B in breast cancer tissues versus normal tissues might be due to the amplification of GOLT1B and altered phosphorylation of its potential transcriptional factors, including JUN and SIN3A. Subsequently, we discovered that GOLT1B potentially regulated the immune microenvironment basing on the finding that its expression was closely related to the tumor microenvironment score and infiltration of immune cells. Moreover, we revealed that GOLT1B might affect the overall survival rates of breast cancer through regulating the immune cell infiltration. Finally, we disclosed the potential pathways involved in the functions of GOLT1B in breast cancer, including metabolism and ECM-receptor interaction pathways. To sum up, we identified GOLT1B as a potential prognostic gene for breast cancer and disclosed its role in regulating the immune microenvironment.

GOLT1B Golgi apparatus immune microenvironment breast cancer prognostic biomarker

SCI

英语

第一 ; 通讯

National Natural Science Foundation of China[82003172] ; Shenzhen Fund for Guangdong Provincial High-level Clinical Key Specialties[SZGSP001] ; Postdoctoral Science Foundation of China[2020M673065] ; Science and Technology Plan of Shenzhen[JCYJ20180306140810282] ; Guangdong Basic and Applied Basic Research Foundation[2019A1515111138]

Oncology

Oncology

WOS:000752611700001

FRONTIERS MEDIA SA

Web of Science

1.Jinan Univ, Southern Univ Sci & Technol,Dept Clin Med Res Ctr, Guangdong Prov Engn Res Ctr Autoimmune Dis Precis, Affiliated Hosp 1,Clin Med Coll 2,Shenzhen People, Shenzhen, Peoples R China
2.Jinan Univ, Affiliated Hosp 1, Guangzhou, Peoples R China
3.Chongqing Med Univ, Chinese Minist Educ, Key Lab Diagnost Med, Chongqing, Peoples R China
4.Chongqing Med Univ, Lab Teaching & Management Ctr, Chongqing, Peoples R China

Liu, Junping,Zhang, Wei,Cai, Wanxia,et al. Multi-Omics Analyses Revealed GOLT1B as a Potential Prognostic Gene in Breast Cancer Probably Regulating the Immune Microenvironment[J]. Frontiers in Oncology,2022,11.

Liu, Junping.,Zhang, Wei.,Cai, Wanxia.,Chen, Yumei.,Cai, Xiaozhong.,...&Dai, Yong.(2022).Multi-Omics Analyses Revealed GOLT1B as a Potential Prognostic Gene in Breast Cancer Probably Regulating the Immune Microenvironment.Frontiers in Oncology,11.

Liu, Junping,et al."Multi-Omics Analyses Revealed GOLT1B as a Potential Prognostic Gene in Breast Cancer Probably Regulating the Immune Microenvironment".Frontiers in Oncology 11(2022).