Simultaneous probing of dual intracellular metabolites (ATP and paramylon) in live microalgae using graphene oxide/aptamer nanocomplex

Kim, Jee Young1; Jin, Cho Rok1; Park, Jaewon2; Kim, Dae Geun3; Kim, Hyun Soo4; Choi, Yoon-E1

2022-03-01

10.1007/s00604-022-05198-5

MICROCHIMICA ACTA   影响因子和分区

0026-3672

1436-5073

The development of an intracellular metabolite imaging platform for live microorganisms has been a challenge in the study of microbes. Herein, we performed metabolite imaging in live microalgal cells using a graphene oxide (GO)/aptamer complex. The properties of the GO were characterized using dynamic light scattering (DLS) and atomic force microscopy (AFM), which were determined to have 140 +/- 3 nm in mean diameter. An ATP-specific aptamer was mixed with GO to form a GO/aptamer complex, and the feasibility of the complex was tested in vitro. The high correlation between the fluorescence intensity and concentration of ATP was observed in the range 0-10 mM. Next, the feasibility of the complex was confirmed in vivo. Under both phototrophic and heterotrophic culture conditions, Euglena gracilis internalized the complex, and bright fluorescence was observed as the aptamer was bound to the target metabolite (ATP). The fluorescence intensity of cells was correlated to the ATP concentration in the cells. Imaging of dual intracellular metabolites (ATP and paramylon) was achieved by simply using two different aptamers (ATP-specific aptamer and paramylon-specific aptamer) together, showing the great potential of the complex as a dual-sensing/imaging platform. In addition, the GO/aptamer complex exhibited low cytotoxicity; the proliferation and viability of E. gracilis cells were not significantly affected by the complex. Our results suggested that this new imaging platform can be efficiently used for detecting dual intracellular metabolites in live microalgal cells.

Metabolite sensing Graphene oxide Dual-imaging Euglena gracilis In vivo detection Aptamers

SCI

英语

其他

NRF (National Research Foundation of Korea) - Korean Government (MSIT)["2019R1A2C2087449","NRF-2021R1A6A1A10045235","NRF2021R1F1A1063455"] ; Korea Environment Industry & Technology Institute (KEITI) through Ecological Imitation-based Environmental Pollution Management Technology Development Project - Korea Ministry Environment (MOE)[2019002790009]

Chemistry

Chemistry, Analytical

WOS:000752355000008

SPRINGER WIEN

CHEMISTRY

2-s2.0-85124219203

Web of Science

1.Korea Univ, Div Environm Sci & Ecol Engn, Seoul 02841, South Korea
2.Southern Univ Sci & Technol, Sch Microelect, Shenzhen 518055, Peoples R China
3.Chonbuk Natl Univ, LED Agribio Fus Technol Res Ctr, Jeonju Si 54596, Jeollabuk Do, South Korea
4.Kwangwoon Univ, Dept Elect Engn, Seoul 01897, South Korea

Kim, Jee Young,Jin, Cho Rok,Park, Jaewon,et al. Simultaneous probing of dual intracellular metabolites (ATP and paramylon) in live microalgae using graphene oxide/aptamer nanocomplex[J]. MICROCHIMICA ACTA,2022,189(3).

Kim, Jee Young,Jin, Cho Rok,Park, Jaewon,Kim, Dae Geun,Kim, Hyun Soo,&Choi, Yoon-E.(2022).Simultaneous probing of dual intracellular metabolites (ATP and paramylon) in live microalgae using graphene oxide/aptamer nanocomplex.MICROCHIMICA ACTA,189(3).

Kim, Jee Young,et al."Simultaneous probing of dual intracellular metabolites (ATP and paramylon) in live microalgae using graphene oxide/aptamer nanocomplex".MICROCHIMICA ACTA 189.3(2022).