Probing the Effect of Ubiquitinated Histone on Mononucleosomes by Translocation Dynamics Study through Solid-State Nanopores

Hu, Rui1; Liu, Cuifang2; Lu, Wenlong1; Wei, Guanghao1; Yu, Dapeng1,3,4; Li, Wei5,6,7; Chen, Ping2,8; Li, Guohong2,9; Zhao, Qing1,10,11

2022

10.1021/acs.nanolett.1c02978

NANO LETTERS   影响因子和分区

1530-6984

1530-6992

Post-translational modifications (PTMs), such as ubiquitination, are critically important in regulating genetic expressions by adjusting the nucleosome stability. A fast and label-free technology inspecting dynamic nucleosome structures can facilitate the interrogation of PTMs effects. Here we leverage the advantages of mechanically stable solid-state nanopores and detect the effect of a ubiquitinated histone on mononucleosomes at the single-molecule level. By comparing the translocation dynamics of natural and cross-linked mononucleosomes, we verified that the nucleosomal DNA unravelled from histones in natural mononucleosomes. Furthermore, we found that a turning point of voltage corresponds to the onset of nucleosome rupture. More importantly, we reveal that ubH2A stabilizes the nucleosome by shifting the turning point to a larger value and investigated the effect of ubiquitination on different histones (ubH2A and ubH2B). These findings open promising possibilities for developing a miniaturized and portable device for the fast screening of PTMs on nucleosomes.

Solid-state nanopore Nucleosome Single-molecule detection Ubiquitination Post-translational modification

SCI ; EI

英语

NI论文

其他

Ministry of Science and Technology of China["2019YFE0114100","2019YFA0707003","2017YFA0504202","2018YFE0203302"] ; National Natural Science Foundation of China[51872007,52103296,32100470,31991161,31630041,32022014,31871290,11874414] ; Beijing Municipal Natural Science Foundation[7202094] ; Joint Laboratory of Offshore Wind Power and Intelligent Energy System[HNKJ20-H88] ; Beijing Municipal Science and Technology Committee[Z201100005320013] ; Chinese Academy of Sciences (CAS) Key Research Program on Frontier Science["QYZDY-SSW-SMC020","QYZDB-SSW-SLH045"] ; CAS Strategic Priority Research Program of Chinese Academy of Sciences[XDB37000000] ; National Laboratory of Biomacromolecules[2020kf02]

Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics

Chemistry, Multidisciplinary ; Chemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied ; Physics, Condensed Matter

WOS:000752904400001

AMER CHEMICAL SOC

20220511580708

Gene expression ; Molecules ; Nanopores

Biology:461.9 ; Nanotechnology:761 ; Organic Compounds:804.1 ; Atomic and Molecular Physics:931.3 ; Solid State Physics:933

MATERIALS SCIENCE

Web of Science

1.Peking Univ, Sch Phys, Ctr Nanooptoelect, State Key Lab Mesoscop Phys & Frontiers Sci, Beijing 100871, Peoples R China
2.Chinese Acad Sci, Inst Biophys, CAS Ctr Excellence Biomacromol, Natl Lab Biomacromol, Beijing 100101, Peoples R China
3.South Univ Sci & Technol China SUSTech, Inst Quantum Sci & Technol, Shenzhen 518055, Peoples R China
4.South Univ Sci & Technol China SUSTech, Dept Phys, Shenzhen 518055, Peoples R China
5.Chinese Acad Sci, Natl Lab Condensed Matter Phys, Beijing 100190, Peoples R China
6.Chinese Acad Sci, Key Lab Soft Matter Phys, Inst Phys, Beijing 100190, Peoples R China
7.Songshan Lake Mat Lab, Dongguan 523808, Guangdong, Peoples R China
8.Capital Med Univ, Sch Basic Med Sci, Adv Innovat Ctr Human Brain Protect, Beijing 100069, Peoples R China
9.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
10.Peking Univ, Yangtze Delta Inst Optoelect, Nantong 226010, Jiangsu, Peoples R China
11.Collaborat Innovat Ctr Quantum Matter, Beijing 100084, Peoples R China

Hu, Rui,Liu, Cuifang,Lu, Wenlong,et al. Probing the Effect of Ubiquitinated Histone on Mononucleosomes by Translocation Dynamics Study through Solid-State Nanopores[J]. NANO LETTERS,2022,22(3):888-895.

Hu, Rui.,Liu, Cuifang.,Lu, Wenlong.,Wei, Guanghao.,Yu, Dapeng.,...&Zhao, Qing.(2022).Probing the Effect of Ubiquitinated Histone on Mononucleosomes by Translocation Dynamics Study through Solid-State Nanopores.NANO LETTERS,22(3),888-895.

Hu, Rui,et al."Probing the Effect of Ubiquitinated Histone on Mononucleosomes by Translocation Dynamics Study through Solid-State Nanopores".NANO LETTERS 22.3(2022):888-895.