Downregulation of sperm-associated antigen 5 inhibits melanoma progression by regulating forkhead box protein M1/A disintegrin and metalloproteinase 17/NOTCH1 signaling

Dang, Lin1; Shi, Cuiping1; Zhang, Qianqian1; Liao, Peiyu1; Wang, Yan2

2022-03-01

10.1080/21655979.2022.2031670

Bioengineered   影响因子和分区

2165-5979

2165-5987

Sperm-associated antigen 5 (SPAG5) has been identified as a driver in several type of cancers. In this study, we aimed to reveal the role of SPAG5 in melanoma and clarify whether FOXM1 (forkhead box protein M1) /ADAM17 (A disintegrin and metalloproteinase 17) /NOTCH1 signaling was involved. The expression of SPAG5 in malignant melanoma (MM) tissues and matched normal tissues was detected using qRT-PCR, immunohistochemistry and Western blotting. Cell viability was tested using CCK-8 (Cell Count Kit-8), colony formation and EdU staining. Cell migration and epithelial to mesenchymal transition (EMT) were measured using transwell chambers and immunofluorescent staining. Cell cycle distribution and tumorigenesis were assessed by flow cytometry and in vivo tumor-bearing experiments, respectively. The results demonstrated that the expression of SPAG5 was increased in MM tissues and cells. Downregulation of SPAG5 inhibited cell viability, migration, invasion and EMT, and induced a G1-phase arrest. In addition, downregulation of SPAG5 decreased the expression of FOXM1, thereafter inhibiting the expression of ADAM17, NOTCH1 and HES1. Furthermore, deletion of SPAG5 expression decreased the tumorigenesis of MM A375 cells. In conclusion, this study demonstrated that SPAG5 was overexpressed in MM. Downregulation of SPAG5 repressed MM cell growth and EMT, which might be induced by inactivation of the FOXM1/ADAM17/NOTCH1 signaling.

Melanoma cell growth migration Sperm-associated antigen 5 forkhead box protein M1

SCI

英语

第一 ; 通讯

Biotechnology & Applied Microbiology

Biotechnology & Applied Microbiology

WOS:000753162700001

TAYLOR & FRANCIS INC

Web of Science

1.Southern Univ Sci & Technol, Affiliated Hosp 1, Clin Med Coll 2, Shenzhen Peoples Hosp,Dept Dermatovenol,Jinan Uni, Shenzhen, Peoples R China
2.Southern Univ Sci & Technol, Affiliated Hosp 1, Jinan Univ, Dept Pathol,Shenzhen Peoples Hosp,Clin Med Coll 2, 1017 Dongmen North Rd, Shenzhen 518020, Peoples R China

Dang, Lin,Shi, Cuiping,Zhang, Qianqian,et al. Downregulation of sperm-associated antigen 5 inhibits melanoma progression by regulating forkhead box protein M1/A disintegrin and metalloproteinase 17/NOTCH1 signaling[J]. Bioengineered,2022,13(3).

Dang, Lin,Shi, Cuiping,Zhang, Qianqian,Liao, Peiyu,&Wang, Yan.(2022).Downregulation of sperm-associated antigen 5 inhibits melanoma progression by regulating forkhead box protein M1/A disintegrin and metalloproteinase 17/NOTCH1 signaling.Bioengineered,13(3).

Dang, Lin,et al."Downregulation of sperm-associated antigen 5 inhibits melanoma progression by regulating forkhead box protein M1/A disintegrin and metalloproteinase 17/NOTCH1 signaling".Bioengineered 13.3(2022).