Mussel-inspired nano-building block assemblies for mimicking extracellular matrix microenvironments with multiple functions

Wang, Zhenming1; Jia, Zhanrong1; Jiang, Yanan1; Li, Pengfei1; Han, Lu1; Lu, Xiong1,2; Ren, Fuzeng3; Wang, Kefeng2; Yuan, Huiping4

2017-09

10.1088/1758-5090/aa7fdc

Biofabrication   影响因子和分区

1758-5082

1758-5090

The assembly of nano-building blocks is an effective way to produce artificial extracellular matrix microenvironments with hierarchical micro/nano structures. However, it is hard to assemble different types of nano-building blocks, to form composite coatings with multiple functions, by traditional layer-by-layer (LbL) self-assembly methods. Inspired by the mussel adhesion mechanism, we developed polydopamine (PDA)-decorated bovine serum albumin microspheres (BSA-MS) and nano-hydroxyapatite (nano-HA), and assembled them to form bioactive coatings with micro/nano structures encapsulating bone morphogenetic protein-2 (BMP-2). First, PDA-decorated nano-HA (nano-pHA) was obtained by oxidative polymerization of dopamine on nano-HA. Second, BMP-2-encapsulated BSA microspheres were prepared through desolvation, and then were also decorated by PDA (pBSA-MS). Finally, the nano-pHA and pBSA-MS were assembled using the adhesive properties of PDA. Bone marrow stromal cell cultures and in vivo implantation, showed that the pHA/pBSA (BMP-2) coatings can promote cell adhesion, proliferation, and benefited for osteoinductivity. PDA decoration was also applied to assemble various functional nanoparticles, such as nano-HA, polystyrene, and Fe3O4 nanoparticles. In summary, this study provides a novel strategy for the assembly of biofunctional nano-building blocks, which surpasses traditional LbL self-assembly of polyelectrolytes, and can find broad applications in bioactive agents delivery or multi-functional coatings.

polydopamine nano-HA BMP-2 self-assembly osteoinductivity

SCI ; EI

英语

其他

Sichuan Province Youth Science and Technology Innovation Team[2016TD0026]

Engineering ; Materials Science

Engineering, Biomedical ; Materials Science, Biomaterials

WOS:000406921000001

IOP PUBLISHING LTD

20173404071457

Body fluids ; Bone ; Cell adhesion ; Cell culture ; Hard coatings ; Hydroxyapatite ; Iron oxides ; Magnetite ; Microspheres ; Molluscs ; Nanocomposites ; Nanoparticles ; Polyelectrolytes ; Protective coatings ; Self assembly

Biological Materials and Tissue Engineering:461.2 ; Biology:461.9 ; Marine Science and Oceanography:471 ; Nanotechnology:761 ; Inorganic Compounds:804.2 ; Coatings and Finishes:813 ; Coating Materials:813.2 ; Organic Polymers:815.1.1 ; Solid State Physics:933 ; Materials Science:951

Web of Science

1.Southwest Jiaotong Univ, Sch Mat Sci & Engn, Key Lab Adv Technol Mat, Minist Educ, Chengdu 610031, Sichuan, Peoples R China
2.Sichuan Univ, Natl Engn Res Ctr Biomat, Genome Res Ctr Biomat, Chengdu 610064, Sichuan, Peoples R China
3.South Univ Sci & Technol, Dept Mat Sci & Engn, Shenzhen 518055, Guangdong, Peoples R China
4.Sichuan Univ, Coll Phys Sci & Technol, Chengdu 610064, Sichuan, Peoples R China

Wang, Zhenming,Jia, Zhanrong,Jiang, Yanan,et al. Mussel-inspired nano-building block assemblies for mimicking extracellular matrix microenvironments with multiple functions[J]. Biofabrication,2017,9(3).

Wang, Zhenming.,Jia, Zhanrong.,Jiang, Yanan.,Li, Pengfei.,Han, Lu.,...&Yuan, Huiping.(2017).Mussel-inspired nano-building block assemblies for mimicking extracellular matrix microenvironments with multiple functions.Biofabrication,9(3).

Wang, Zhenming,et al."Mussel-inspired nano-building block assemblies for mimicking extracellular matrix microenvironments with multiple functions".Biofabrication 9.3(2017).