Crystal structure of the cytokinin-producing enzyme “lonely guy” (LOG) from Mycobacterium tuberculosis

Shang，Le1; Li，Guobao1; Lin，Qiao2; Ou，Min1; Liang，Juan1; Xiao，Guohui1; Wang，Zhaoqin1; Cui，Sheng3; Zhang，Tianyu4; Liu，Lei1; Zhang，Guoliang1

2022-04-02

10.1016/j.bbrc.2022.01.103

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   影响因子和分区

0006-291X

1090-2104

Mycobacterium tuberculosis (Mtb) is an extremely successful intracellular pathogen that cause a large number of death worldwide. It is interesting that this non-phytopathogen can synthesize cytokinin by “lonely guy” (LOG) protein. The cytokinin biosynthesis pathway in Mtb is not clear. Here we determined the crystal structure of LOG from Mtb (MtLOG) at a high resolution of 1.8 Å. MtLOG exists as dimer which belongs to type-I LOG and shows a typical α-β Rossmann fold. Like other LOGs, MtLOG also contains a conserved “PGGXGTXXE” motif that contributes to the formation of an active site. For the first time, we found that the MtLOG binds to Mg in the negative potential pocket. According to the docking result, we found that Arg78, Arg98 and Tyr162 should be the key amino acid involved in substrate binding. Our findings provide a structural basis for cytokinin study in Mtb and will play an important role in design and development of enzyme inhibitors.

Cytokinin LOG Mycobacterium tuberculosis

英语

第一 ; 通讯

BIOLOGY & BIOCHEMISTRY

2-s2.0-85124323587

Scopus

1.National Clinical Research Center for Infectious Diseases,Guangdong Provincial Clinical Research Center for Tuberculosis,Shenzhen Third People's Hospital,Southern University of Science and Technology,Shenzhen,518112,China
2.Department of Traditional Chinese Medicine,The Baoan People's Hospital of Shenzhen,Shenzhen University,Shenzhen,518101,China
3.Institute of Pathogen Biology,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing,100730,China
4.State Key Laboratory of Respiratory Disease,Guangzhou Institutes of Biomedicine and Health,Chinese Academy of Sciences,Guangzhou,510700,China

Shang，Le,Li，Guobao,Lin，Qiao,等. Crystal structure of the cytokinin-producing enzyme “lonely guy” (LOG) from Mycobacterium tuberculosis[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2022,598:113-118.

Shang，Le.,Li，Guobao.,Lin，Qiao.,Ou，Min.,Liang，Juan.,...&Zhang，Guoliang.(2022).Crystal structure of the cytokinin-producing enzyme “lonely guy” (LOG) from Mycobacterium tuberculosis.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,598,113-118.

Shang，Le,et al."Crystal structure of the cytokinin-producing enzyme “lonely guy” (LOG) from Mycobacterium tuberculosis".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 598(2022):113-118.