Multicolor Super-resolution Fluorescence Microscopy with Blue and Carmine Small Photoblinking Polymer Dots

Chen, Xuanze1,2; Liu, Zhihe3,4; Li, Rongqin1,2; Shan, Chunyan1,2; Zeng, Zhiping5; Xue, Boxin1,2; Yuan, Weihong1,2; Mo, Chi1,2; Xi, Peng1,2; Wu, Changfeng3; Sun, Yujie1,2

2017-08

10.1021/acsnano.7b02893

ACS Nano   影响因子和分区

1936-0851

1936-086X

Advances in the development of small photoblinking semiconducting polymer dots (Pdots) have attracted great interest for use in super-resolution microscopy. However, multicolor super-resolution imaging using conventional small photoblinking Pdots remains a challenge due to their limited color choice, broad emission spectrum, and heavy spectrum crosstalk. Here, we introduce two types of small photoblinking Pdots with different colors and relatively narrow emission spectra: blue PFO Pdots and carmine PFTBTS Pdots for blinking-based statistical nanoscopy. Both of these probes feature ultrahigh single-particle brightness, very strong photostability, superior biocompatibility, and robust fluorescence fluctuation. In addition, these small photoblinking Pdots serve as excellent labels for dual-color super resolution optical fluctuation imaging (SOFT) of specific subcellular structures, indicating their promise for long-term multicolor SOFI nanoscopy with high spatiotemporal resolution.

multicolor super-resolution imaging super-resolution optical fluctuation imaging blue and carmine small polymer dots photoblinking bioimaging

SCI ; EI

英语

NI期刊

通讯

"863" Program[SS2015AA020406]

Chemistry ; Science & Technology - Other Topics ; Materials Science

Chemistry, Multidisciplinary ; Chemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary

WOS:000408520900058

AMER CHEMICAL SOC

20173504104188

Biocompatibility ; Color ; Emission spectroscopy ; Fluorescence ; Fluorescence microscopy ; Polymers

Immunology:461.9.1 ; Light/Optics:741.1 ; Polymeric Materials:815.1 ; Quantum Theory; Quantum Mechanics:931.4

Web of Science

1.Peking Univ, Sch Life Sci, State Key Lab Membrane Biol, Biodynam Opt Imaging Ctr BIOPIC, Beijing 100871, Peoples R China
2.Peking Univ, Dept Biomed Engn, Coll Engn, Beijing 100871, Peoples R China
3.Southern Univ Sci & Technol, Dept Biomed Engn, Shenzhen 510855, Guangdong, Peoples R China
4.Jilin Univ, Coll Elect Sci & Engn, State Key Lab Integrated Optoelect, Changchun 130012, Jilin, Peoples R China
5.Fuzhou Univ, Coll Phys & Informat Engn, Fuzhou 350116, Fujian, Peoples R China

Chen, Xuanze,Liu, Zhihe,Li, Rongqin,et al. Multicolor Super-resolution Fluorescence Microscopy with Blue and Carmine Small Photoblinking Polymer Dots[J]. ACS Nano,2017,11(8):8084-8091.

Chen, Xuanze.,Liu, Zhihe.,Li, Rongqin.,Shan, Chunyan.,Zeng, Zhiping.,...&Sun, Yujie.(2017).Multicolor Super-resolution Fluorescence Microscopy with Blue and Carmine Small Photoblinking Polymer Dots.ACS Nano,11(8),8084-8091.

Chen, Xuanze,et al."Multicolor Super-resolution Fluorescence Microscopy with Blue and Carmine Small Photoblinking Polymer Dots".ACS Nano 11.8(2017):8084-8091.