Real-Time Imaging of Endocytosis and Intracellular Trafficking of Semiconducting Polymer Dots

Han, Yuping1; Li, Xiaoming5; Chen, Haobin4; Hu, Xingjie2,3; Luo, Yao1,2,3; Wang, Ting1,2,3; Wang, Zejun2,3; Li, Qian2,3; Fan, Chunhai2,3,5; Shi, Jiye2,3,6; Wang, Lihua2,3; Zhao, Yun1; Wu, Changfeng4; Chen, Nan2,3

2017-06-28

10.1021/acsami.7b05662

ACS Applied Materials & Interfaces   影响因子和分区

1944-8244

Semiconducting polymer dots (Pdots) have shown great promise in biomedical applications, including biosensing, drug delivery, and live imaging of cells and biomolecules. Insight into the mechanism and regulation of cellular uptake and intracellular metabolism. of Pdots is important for the development of superior Pdots-based theranostic nanoconjugates. Herein, we performed real-time imaging of endocytosis and intracellular trafficking of a type of fluorescent Pdots that showed excellent biocompatibility in various types of cells. The endocytic routes and kinetics of Pdots were differently regulated in distinct cell types. Following endocytosis, Pdots were transported in vesicles along micro tubule and destined for lysosomes. Furthermore, our results revealed exosome-mediated extracellular release of Pdots and have tracked the dynamic process at the single particle level. These results provide new insight into the design of more effective and selective imaging probes as well as drug carriers.

fluorescent polymer dots endocytosis intracellular trafficking theranostic nanoparticles live cell imaging

SCI ; EI

英语

通讯

Key Research Program of Frontier Sciences, CAS[QYZDJ-SSW-SLH031] ; Key Research Program of Frontier Sciences, CAS[QYZDJ-SSW-SLH019]

Science & Technology - Other Topics ; Materials Science

Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary

WOS:000404807200017

AMER CHEMICAL SOC

20172703879392

Biocompatibility ; Cells ; Controlled drug delivery ; Cytology ; Fluorescence ; Functional polymers ; Medical applications ; Molecular biology ; Targeted drug delivery

Bioengineering and Biology:461 ; Light/Optics:741.1 ; Polymeric Materials:815.1

Web of Science

1.Sichuan Univ, Coll Life Sci, Chengdu 610064, Peoples R China
2.Chinese Acad Sci, Div Phys Biol, Shanghai Synchrotron Radiat Facil, CAS Key Lab Interfacial Phys & Technol,Shanghai I, Shanghai 201800, Peoples R China
3.Chinese Acad Sci, Bioimaging Ctr, Shanghai Synchrotron Radiat Facil, CAS Key Lab Interfacial Phys & Technol,Shanghai I, Shanghai 201800, Peoples R China
4.Southern Univ Sci & Technol, Dept Biomed Engn, Shenzhen 518055, Guangdong, Peoples R China
5.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China
6.UCB Pharma, 208 Bath Rd, Slough SL1 3WE, Berks, England

Han, Yuping,Li, Xiaoming,Chen, Haobin,et al. Real-Time Imaging of Endocytosis and Intracellular Trafficking of Semiconducting Polymer Dots[J]. ACS Applied Materials & Interfaces,2017,9(25):21200-21208.

Han, Yuping.,Li, Xiaoming.,Chen, Haobin.,Hu, Xingjie.,Luo, Yao.,...&Chen, Nan.(2017).Real-Time Imaging of Endocytosis and Intracellular Trafficking of Semiconducting Polymer Dots.ACS Applied Materials & Interfaces,9(25),21200-21208.

Han, Yuping,et al."Real-Time Imaging of Endocytosis and Intracellular Trafficking of Semiconducting Polymer Dots".ACS Applied Materials & Interfaces 9.25(2017):21200-21208.