Screening on-chip fabricated nanoparticles for penetrating the blood-brain barrier

Hou, Qinghong1,2,3; Zhu, Lina1; Wang, Le2; Liu, Xiaoyan2; Xiao, Feng2; Xie, Yangzhouyun2; Zheng, Wenfu3; Jiang, Xingyu2

2022-02-01

10.1039/d1nr05825h

Nanoscale   影响因子和分区

2040-3364

2040-3372

The inability of drugs to cross the blood-brain barrier (BBB) makes it difficult to treat diseases in the central nervous system. It is known that peptides with or without specific receptors on the BBB showed different or even controversial neuron targeting capability in different reports. So, it is necessary to clarify how these peptides work as targeting molecules in the central nervous system. Herein, we evaluate and compare the performance of 6 kinds of peptides with (T7, D-T7, and GSH) or without (TGN, CGN, and TAT) BBB-specific receptors by conjugating these peptides on lipids to serve as a shell to encapsulate a core of PLGA and lamotrigine to form nanoparticles for targeted epilepsy therapy. In vitro assay shows that the TAT-modified nanoparticles show the highest internalization efficacy in the BBB model cell line bEnd center dot 3 cells and hippocampal neurons. By contrast, experiments in mice show that the D-T7-modified nanoparticles have the highest brain targeting and epilepsy therapeutic efficiency. Thus, our experiments uncover the different performances of the 6 peptides at different levels (in vitro and in vivo), which is insightful for developing novel delivery systems for treating diseases in the central nervous system.

SCI ; EI

英语

通讯

National Natural Science Foundation of China[81730051,32071390,22104050] ; Shenzhen Science and Technology Program[KQTD20190929172743294] ; National Key R&D Program of China[2018YFA0902600] ; Chinese Academy of Sciences[QYZDJ-SSW-SLH039] ; Shenzhen Bay Laboratory[SZBL2019062801004] ; Shenzhen Key Laboratory of Smart Healthcare Engineering[ZDSYS20200811144003009]

Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics

Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied

WOS:000754751000001

ROYAL SOC CHEMISTRY

20221011748932

Blood ; Cell culture ; Diagnosis ; Mammals ; Nanoparticles ; Neurology ; Targeted drug delivery

Biological Materials and Tissue Engineering:461.2 ; Medicine and Pharmacology:461.6 ; Biology:461.9 ; Nanotechnology:761 ; Solid State Physics:933

Web of Science

1.Tianjin Univ, Sch Sci, Dept Chem, Tianjin 300072, Peoples R China
2.Southern Univ Sci & Technol, Dept Biomed Engn, 1088 Xueyuan Rd, Shenzhen 518055, Guangdong, Peoples R China
3.Natl Ctr NanoSci & Technol, CAS Key Lab Biol Effects Nanomat & Nanosafety, Beijing 100190, Peoples R China

Hou, Qinghong,Zhu, Lina,Wang, Le,et al. Screening on-chip fabricated nanoparticles for penetrating the blood-brain barrier[J]. Nanoscale,2022,14:3234-3241.

Hou, Qinghong.,Zhu, Lina.,Wang, Le.,Liu, Xiaoyan.,Xiao, Feng.,...&Jiang, Xingyu.(2022).Screening on-chip fabricated nanoparticles for penetrating the blood-brain barrier.Nanoscale,14,3234-3241.

Hou, Qinghong,et al."Screening on-chip fabricated nanoparticles for penetrating the blood-brain barrier".Nanoscale 14(2022):3234-3241.