Regulation of cytokinin biosynthesis using PtRD26(pro)-IPT module improves drought tolerance through PtARR10-PtYUC4/5-mediated reactive oxygen species removal in Populus

Wang, Hou-Ling1; Yang, Qi1; Tan, Shuya1; Wang, Ting1; Zhang, Yi2; Yang, Yanli1; Yin, Weilun1; Xia, Xinli1; Guo, Hongwei2; Li, Zhonghai1

2022-02-01

10.1111/jipb.13218

Journal of Integrative Plant Biology   影响因子和分区

1672-9072

1744-7909

Drought is a critical environmental factor which constrains plant survival and growth. Genetic engineering provides a credible strategy to improve drought tolerance of plants. Here, we generated transgenic poplar lines expressing the isopentenyl transferase gene (IPT) under the driver of PtRD26 promoter (PtRD26(pro)-IPT). PtRD26 is a senescence and drought-inducible NAC transcription factor. PtRD26(pro)-IPT plants displayed multiple phenotypes, including improved growth and drought tolerance. Transcriptome analysis revealed that auxin biosynthesis pathway was activated in the PtRD26(pro)-IPT plants, leading to an increase in auxin contents. Biochemical analysis revealed that ARABIDOPSIS RESPONSE REGULATOR10 (PtARR10), one of the type-B ARR transcription factors in the cytokinin pathway, was induced in PtRD26(pro)-IPT plants and directly regulated the transcripts of YUCCA4 (PtYUC4) and YUCCA5 (PtYUC5), two enzymes in the auxin biosynthesis pathway. Overexpression of PtYUC4 enhanced drought tolerance, while simultaneous silencing of PtYUC4/5 evidently attenuated the drought tolerance of PtRD26(pro)-IPT plants. Intriguingly, PtYUC4/5 displayed a conserved thioredoxin reductase activity that is required for drought tolerance by deterring reactive oxygen species accumulation. Our work reveals the molecular basis of cytokinin and auxin interactions in response to environmental stresses, and shed light on the improvement of drought tolerance without a growth penalty in trees by molecular breeding.

auxin cytokinin drought tolerance leaf senescence PtARR PtYUC ROS

SCI

英语

其他

National Natural Science Foundation of China[32170345,31970196,32011540381,31900173,31770649] ; Open Fund of State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry[TGB2021007] ; National Key Research and Development Program of China["2019YFA0903904","BJFU2021YJRC00600K"]

Biochemistry & Molecular Biology ; Plant Sciences

Biochemistry & Molecular Biology ; Plant Sciences

WOS:000753925200001

WILEY

Web of Science

1.Beijing Forestry Univ, Coll Biol Sci & Technol, Natl Engn Lab Tree Breeding, Beijing 100083, Peoples R China
2.Southern Univ Sci & Technol SUSTech, Key Lab Mol Design Plant Cell Factory, Dept Biol, Guangdong Higher Educ Inst, Shenzhen 518055, Peoples R China

Wang, Hou-Ling,Yang, Qi,Tan, Shuya,et al. Regulation of cytokinin biosynthesis using PtRD26(pro)-IPT module improves drought tolerance through PtARR10-PtYUC4/5-mediated reactive oxygen species removal in Populus[J]. Journal of Integrative Plant Biology,2022.

Wang, Hou-Ling.,Yang, Qi.,Tan, Shuya.,Wang, Ting.,Zhang, Yi.,...&Li, Zhonghai.(2022).Regulation of cytokinin biosynthesis using PtRD26(pro)-IPT module improves drought tolerance through PtARR10-PtYUC4/5-mediated reactive oxygen species removal in Populus.Journal of Integrative Plant Biology.

Wang, Hou-Ling,et al."Regulation of cytokinin biosynthesis using PtRD26(pro)-IPT module improves drought tolerance through PtARR10-PtYUC4/5-mediated reactive oxygen species removal in Populus".Journal of Integrative Plant Biology (2022).