SWELLMAP 2, a phyB-Interacting Splicing Factor, Negatively Regulates Seedling Photomorphogenesis in Arabidopsis

Yan, Tingting1,2; Heng, Yueqin2; Wang, Wenwei2; Li, Jian2; Deng, Xing Wang2,3

2022-02-10

10.3389/fpls.2022.836519

Frontiers in Plant Science   影响因子和分区

1664-462X

Light-triggered transcriptome reprogramming is critical for promoting photomorphogenesis in Arabidopsis seedlings. Nonetheless, recent studies have shed light on the importance of alternative pre-mRNA splicing (AS) in photomorphogenesis. The splicing factors splicing factor for phytochrome signaling (SFPS) and reduced red-light responses in cry1cry2 background1 (RRC1) are involved in the phytochrome B (phyB) signaling pathway and promote photomorphogenesis by controlling pre-mRNA splicing of light- and clock-related genes. However, splicing factors that serve as repressors in phyB signaling pathway remain unreported. Here, we report that the splicing factor SWELLMAP 2 (SMP2) suppresses photomorphogenesis in the light. SMP2 physically interacts with phyB and colocalizes with phyB in photobodies after light exposure. Genetic analyses show that SMP2 antagonizes phyB signaling to promote hypocotyl elongation in the light. The homologs of SMP2 in yeast and human belong to second-step splicing factors required for proper selection of the 3' splice site (3'SS) of an intron. Notably, SMP2 reduces the abundance of the functional REVEILLE 8 a (RVE8a) form, probably by determining the 3'SS, and thereby inhibits RVE8-mediated transcriptional activation of clock genes containing evening elements (EE). Finally, SMP2-mediated reduction of functional RVE8 isoform promotes phytochrome interacting factor 4 (PIF4) expression to fine-tune hypocotyl elongation in the light. Taken together, our data unveil a phyB-interacting splicing factor that negatively regulates photomorphogenesis, providing additional information for further mechanistic investigations regarding phyB-controlled AS of light- and clock-related genes.

light signaling photomorphogenesis phytochrome B alternative pre-mRNA splicing splicing factor

SCI

英语

通讯

Plant Sciences

Plant Sciences

WOS:000760979600001

FRONTIERS MEDIA SA

Web of Science

1.Harbin Inst Technol, Harbin, Peoples R China
2.Southern Univ Sci & Technol, Inst Plant & Food Sci, Sch Life Sci, Dept Biol,Key Lab Mol Design Plant Cell Factory,G, Shenzhen, Peoples R China
3.Peking Univ, Peking Tsinghua Ctr Life Sci, Sch Adv Agr Sci & Sch Life Sci, State Key Lab Prot & Plant Gene Res, Beijing, Peoples R China

Yan, Tingting,Heng, Yueqin,Wang, Wenwei,et al. SWELLMAP 2, a phyB-Interacting Splicing Factor, Negatively Regulates Seedling Photomorphogenesis in Arabidopsis[J]. Frontiers in Plant Science,2022,13.

Yan, Tingting,Heng, Yueqin,Wang, Wenwei,Li, Jian,&Deng, Xing Wang.(2022).SWELLMAP 2, a phyB-Interacting Splicing Factor, Negatively Regulates Seedling Photomorphogenesis in Arabidopsis.Frontiers in Plant Science,13.

Yan, Tingting,et al."SWELLMAP 2, a phyB-Interacting Splicing Factor, Negatively Regulates Seedling Photomorphogenesis in Arabidopsis".Frontiers in Plant Science 13(2022).