Silk Sericin Activates Mild Immune Response and Increases Antibody Production

Zhang, Yuan1,2,4; Shi, Na1,2,3; He, Lun1,2,3; Wang, Shanshan1,2,3; Li, Xin1,2,3; Lu, Shenzhou5; Zhang, Qiqing4,6; Niu, Haitao1,2,3

2021-12-01

10.1166/jbn.2021.3206

Journal of Biomedical Nanotechnology   影响因子和分区

1550-7033

1550-7041

To clarify whether nanoparticles of silk sericin (SS) and silk fibroin (SF) can induce inflammation and immune responses, we analyzed splenocyte proliferation, apoptosis and cytokine release to identify the effects of SS and SF on mouse splenocytes in vitro. We implanted mice with SS and SF through intraperitoneal, intramuscular, and subcutaneous routes to evaluate the innate and adaptive immune response to SS and SF in vivo. Cytokines in the serum and spleen were analyzed by Luminex and antibody array. Antigen-specific antibodies were evaluated by enzyme-linked immunosorbent assay (ELISA) at week 1 and 5 after implantation. Distinct cell populations in the spleen and bone marrow were analyzed by flow cytometry. SS suppressed the proliferation of splenocytes and CD11b(+)CD27(-) NK cells, induced splenocyte apoptosis, and increased interleukin-1 beta (IL-1 beta) and tumor necrosis factor-alpha (TNF-alpha) in the culture supernatant. SF suppressed splenocyte proliferation, induced splenocyte apoptosis, and increased the titer of TNF-alpha in culture supernatants. At both week 1 and 5 after implantation with SS, mouse serum interleukin-1 alpha (IL-1 alpha) and keratinocyte chemoattractant (KC) were decreased, SS-specific antibody was increased, the proportion of bone marrow CD4(+) T cells was increased, and the proportion of splenic neutrophils was decreased. At week 5 after subcutaneous implantation with SF, mouse serum IL-1 alpha, and splenic IL-6, TIMP-1, IL-4, MCP-1, IFN-beta, TCA-3, TNF-alpha, and IL-17 were decreased. SS was able to induce a mild immune response, as evidenced by CD4(+) T cell activation, splenocyte apoptosis, and antigen-specific antibody secretion. Comparatively, SF had low immunogenicity and anti-inflammatory properties.

Silk Fibroin Silk Sericin Immunogenicity Inflammatory Response

SCI

英语

通讯

National Key R&D Programs of China[2017YFC1103603]

Science & Technology - Other Topics ; Materials Science

Nanoscience & Nanotechnology ; Materials Science, Biomaterials

WOS:000756427800013

AMER SCIENTIFIC PUBLISHERS

Web of Science

1.Jinan Univ, Sch Med, Guangzhou 510632, Peoples R China
2.Jinan Univ, Inst Lab Anim Sci, Guangzhou 510632, Peoples R China
3.Jinan Univ, Guangzhou Key Lab Germ Free Mice & Applicat Micro, Guangzhou 510632, Peoples R China
4.Fujian Bote Biotechnol Co Ltd, Fuzhou 350013, Fujian, Peoples R China
5.Soochow Univ, Coll Text & Clothing Engn, Natl Engn Lab Modern Silk, Suzhou 215123, Peoples R China
6.Southern Univ Sci & Technol, Affiliated Hosp 1, Jinan Univ,Inst Biomed Engn, Clin Med Coll 2,Shenzhen Peoples Hosp, Shenzhen 518020, Guangdong, Peoples R China

Zhang, Yuan,Shi, Na,He, Lun,et al. Silk Sericin Activates Mild Immune Response and Increases Antibody Production[J]. Journal of Biomedical Nanotechnology,2021,17(12).

Zhang, Yuan.,Shi, Na.,He, Lun.,Wang, Shanshan.,Li, Xin.,...&Niu, Haitao.(2021).Silk Sericin Activates Mild Immune Response and Increases Antibody Production.Journal of Biomedical Nanotechnology,17(12).

Zhang, Yuan,et al."Silk Sericin Activates Mild Immune Response and Increases Antibody Production".Journal of Biomedical Nanotechnology 17.12(2021).