南方科技大学知识苑(SUSTech KC): Simultaneous detection of Zika, chikungunya, dengue, yellow fever, West Nile, and Japanese encephalitis viruses by a two-tube multiplex real-time RT-PCR assay

题名	Simultaneous detection of Zika, chikungunya, dengue, yellow fever, West Nile, and Japanese encephalitis viruses by a two-tube multiplex real-time RT-PCR assay
作者	Xu, Zhixiang 1,2,3; Peng, Yun 1,2; Yang, Minghui 1; Li, Xiaohe 1,2; Wang, Jun 1,2 ; Zou, Rongrong 1,2; Liang, Jinhu1,2 ; Fang, Shisong 3; Liu, Yingxia 1,2; Yang, Yang 1,2
通讯作者	Yang, Yang
发表日期	2022-02-01
DOI	10.1002/jmv.27658
发表期刊	JOURNAL OF MEDICAL VIROLOGY 影响因子和分区
ISSN	0146-6615
EISSN	1096-9071
摘要	Due to the concurrent prevalence and increasing risk of coinfection of the clinically important Arboviruses, timely and accurate differential diagnosis is important for clinical management and the epidemiological investigation. A two-tube multiplex real-time reverse transcription-polymerase chain reaction (RT-PCR) assay for the simultaneous detection of Zika virus (ZIKV), chikungunya virus (CHIKV), dengue virus (DENV), yellow fever virus (YFV), West Nile virus (WNV), and Japanese encephalitis virus (JEV) was developed and optimized with high specificity and sensitivity. The detection limit for all the six viruses could reach as low as five genome equivalent copies and 2.8 x 10(-)(3) tissue culture infectious doses (TCID50) for ZIKV, YFV, CHIKV and 2.8 x 10(-2) TCID50 for JEV per reaction, with high accuracy and precision (R-2 > 0.99). The coefficient of variation of intra-assay and inter-assay for our quantitative reverse transcription-polymerase chain reaction (qRT-PCR) assay was low, and the obtained positive rates ad C-t values of this assay were comparable with singleplex commercial kits. Moreover, the multiplex qRT-PCR assay was able to detect possible co-infections without competitive inhibition of target viral genomes. In conclusion, our rapid, sensitive, cost-effective multiplex qRT-PCR will be of great use for differential diagnosis in a clinical setting and epidemiological investigation during surveillance.
关键词	arbovirus coinfection detection differential diagnosis multiplex qRT-PCR
相关链接	[来源记录]
收录类别	SCI
语种	英语
学校署名	第一 ; 通讯
资助项目	Shenzhen Science and Technology Research and Development Project["JCYJ20180228162201541","JSGG20201103153802007"] ; National Natural Science Foundation of China[81802004]
WOS研究方向	Virology
WOS类目	Virology
WOS记录号	WOS:000758879600001
出版者	WILEY
ESI学科分类	MICROBIOLOGY
来源库	Web of Science
引用统计	被引频次[WOS]：5
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/291079
专题	南方科技大学第二附属医院南方科技大学第一附属医院
作者单位	1.Southern Univ Sci & Technol, Shenzhen Peoples Hosp 3, Natl Clin Res Ctr Infect Dis, Shenzhen Key Lab Pathogen & Immun,Hosp Affiliated, Shenzhen, Peoples R China 2.Univ Chinese Acad Sci, Savid Med Sch, Beijing, Peoples R China 3.Shenzhen Ctr Dis Control & Prevent, Shenzhen, Peoples R China
第一作者单位	南方科技大学第二附属医院; 南方科技大学第一附属医院
通讯作者单位	南方科技大学第二附属医院; 南方科技大学第一附属医院
第一作者的第一单位	南方科技大学第二附属医院; 南方科技大学第一附属医院
推荐引用方式 GB/T 7714	Xu, Zhixiang,Peng, Yun,Yang, Minghui,et al. Simultaneous detection of Zika, chikungunya, dengue, yellow fever, West Nile, and Japanese encephalitis viruses by a two-tube multiplex real-time RT-PCR assay[J]. JOURNAL OF MEDICAL VIROLOGY,2022.
APA	Xu, Zhixiang.,Peng, Yun.,Yang, Minghui.,Li, Xiaohe.,Wang, Jun.,...&Yang, Yang.(2022).Simultaneous detection of Zika, chikungunya, dengue, yellow fever, West Nile, and Japanese encephalitis viruses by a two-tube multiplex real-time RT-PCR assay.JOURNAL OF MEDICAL VIROLOGY.
MLA	Xu, Zhixiang,et al."Simultaneous detection of Zika, chikungunya, dengue, yellow fever, West Nile, and Japanese encephalitis viruses by a two-tube multiplex real-time RT-PCR assay".JOURNAL OF MEDICAL VIROLOGY (2022).