南方科技大学知识苑(SUSTech KC): P. aeruginosa biofilm activates the NLRP3 inflammasomes in vitro

题名	P. aeruginosa biofilm activates the NLRP3 inflammasomes in vitro
作者	Tan，Qi 1; Ai，Qing 1; He，Yu 1; Li，Fang 1; Yu，Jialin 1,2
通讯作者	Yu，Jialin
发表日期	2022-03-01
DOI	10.1016/j.micpath.2021.105379
发表期刊	MICROBIAL PATHOGENESIS 影响因子和分区
ISSN	0882-4010
EISSN	1096-1208
卷号	164
摘要	The ability of P.aeruginosa to form biofilms renders common treatments inefficient, thereby promoting chronic infection. Inflammasomes activate caspase-1, which is important for the maturation of IL-1β and IL-18 and evoke an inflammatory response. We aimed to investigate the activation of inflammasomes induced by P.aeruginosa biofilm. THP-1 cells were mock-infected or infected with PAO1 biofilms. Protein levels of caspase-1 p20, pro-caspase-1, caspase-4 p20, and pro-caspase-4 in THP-1 macrophages were determined by Western blotting. The expression of NLRC4 and NLRP3 was measured by RT-PCR. The production of IL-1β and IL-18 was monitored using ELISA. P. aeruginosa biofilm significantly elevated caspase-1 levels, and decreased NLRC4 levels. Additionally, caspase-4 and NLRP3 levels were significantly increased. P.aeruginosa biofilm significantly enhanced IL-1β and IL-18 production. We concluded that P. aeruginosa biofilm induced the production of IL-1β and IL-18, possibly via NLRP3 inflammasomes, rather than NLRC4 inflammasomes.
关键词	Biofilm Inflammasome NLRP3 Pseudomonas aeruginosa
相关链接	[Scopus记录]
收录类别	SCI
语种	英语
学校署名	通讯
资助项目	Ministry of Education Key Laboratory of Child Development and Disorders[YBRP-2021XX] ; National Natural Science Foundation of China[81401236] ; Science and health project of Chongqing Health Commission[2021MSXM202] ; project of Chongqing Educational Commission[KJQN201800410]
WOS研究方向	Immunology ; Microbiology
WOS类目	Immunology ; Microbiology
WOS记录号	WOS:000788138500006
出版者	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
ESI学科分类	IMMUNOLOGY
Scopus记录号	2-s2.0-85124263384
来源库	Scopus
引用统计	被引频次[WOS]：2
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/291201
专题	南方科技大学南方科技大学医院
作者单位	1.Department of Neonatology,Children's Hospital of Chongqing Medical University,Chongqing Key Laboratory of Pediatrics,Ministry of Education Key Laboratory of Child Development and Disorders,National Clinical Research Center for Child Health and Disorders,China International Science and Technology Cooperation Base of Child Development and Critical Disorders,Chongqing,China 2.The Third Affiliated Hospital of Southern University of Science and Technology,Shenzhen,China
通讯作者单位	南方科技大学
推荐引用方式 GB/T 7714	Tan，Qi,Ai，Qing,He，Yu,et al. P. aeruginosa biofilm activates the NLRP3 inflammasomes in vitro[J]. MICROBIAL PATHOGENESIS,2022,164.
APA	Tan，Qi,Ai，Qing,He，Yu,Li，Fang,&Yu，Jialin.(2022).P. aeruginosa biofilm activates the NLRP3 inflammasomes in vitro.MICROBIAL PATHOGENESIS,164.
MLA	Tan，Qi,et al."P. aeruginosa biofilm activates the NLRP3 inflammasomes in vitro".MICROBIAL PATHOGENESIS 164(2022).