中文版 | English
题名

Efficient genome editing of genes involved in neural crest development using the CRISPR/Cas9 system in Xenopus embryos

作者
通讯作者Chen, Yonglong; Zhao, Hui
发表日期
2016-03-31
DOI
发表期刊
ISSN
2045-3701
卷号6
摘要

Background: The RNA guided CRISPR/Cas9 nucleases have been proven to be effective for gene disruption in various animal models including Xenopus tropicalis. The neural crest (NC) is a transient cell population during embryonic development and contributes to a large variety of tissues. Currently, loss-of-function studies on NC development in X. tropicalis are largely based on morpholino antisense oligonucleotide. It is worthwhile establishing targeted gene knockout X. tropicails line using CRISPR/Cas9 system to study NC development.

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相关链接[来源记录]
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语种
英语
学校署名
通讯
资助项目
Shenzhen Key Laboratory of Cell Microenvironment[ZDSYS20140509142721429]
WOS研究方向
Biochemistry & Molecular Biology
WOS类目
Biochemistry & Molecular Biology
WOS记录号
WOS:000373193000001
出版者
来源库
Web of Science
引用统计
被引频次[WOS]:13
成果类型期刊论文
条目标识符http://sustech.caswiz.com/handle/2SGJ60CL/29695
专题生命科学学院_生物系
南方科技大学医学院
作者单位
1.Chinese Univ Hong Kong, Sch Biomed Sci, Key Lab Regenerat Med, Minist Educ,Fac Med, Hong Kong, Hong Kong, Peoples R China
2.South Univ Sci & Technol China, Shenzhen Key Lab Cell Microenvironm, Dept Biol, Shenzhen 518055, Guangdong, Peoples R China
3.NCI, Adv Biomed Comp Ctr, NIH, Frederick, MD 21702 USA
4.Ji Nan Univ, Key Lab Regenerat Med, Minist Educ, Guangzhou 510632, Guangdong, Peoples R China
5.KIZ CUHK Joint Lab Bioresources & Mol Res Common, Hong Kong, Hong Kong, Peoples R China
6.Ji Nan Univ, Coll Life Sci & Technol, Dept Dev & Regenerat Biol, Guangzhou 510632, Guangdong, Peoples R China
通讯作者单位生物系;  南方科技大学医学院
推荐引用方式
GB/T 7714
Liu, Zhongzhen,Cheng, Tina Tsz Kwan,Shi, Zhaoying,et al. Efficient genome editing of genes involved in neural crest development using the CRISPR/Cas9 system in Xenopus embryos[J]. Cell and Bioscience,2016,6.
APA
Liu, Zhongzhen.,Cheng, Tina Tsz Kwan.,Shi, Zhaoying.,Liu, Ziran.,Lei, Yong.,...&Zhao, Hui.(2016).Efficient genome editing of genes involved in neural crest development using the CRISPR/Cas9 system in Xenopus embryos.Cell and Bioscience,6.
MLA
Liu, Zhongzhen,et al."Efficient genome editing of genes involved in neural crest development using the CRISPR/Cas9 system in Xenopus embryos".Cell and Bioscience 6(2016).
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