An In Vivo Fluorescence Resonance Energy Transfer-Based Imaging Platform for Targeted Drug Discovery and Cancer Therapy

Huang, Shigao1; Jiang, Cheng2,3; Mughal, Muhammad Jameel4; Wang, Guanyu5,6; Xing, Fuqiang5,7,8

2022-02-14

10.3389/fbioe.2022.839078

Frontiers in Bioengineering and Biotechnology   影响因子和分区

2296-4185

In the present study, an efficient in vivo drug screening platform is established based on FRET technique. We transfected cancer cells with FRET-based caspase-3 (C3) sensor and validated the cell lines by detecting the change in FRET signal caused by the in vitro drug-induced cell apoptosis. Furthermore, the C3 expressing cancer cells were then injected into zebrafish embryos and nude mice to establish the corresponding in vivo xenograft models. We found that cancer cell lines expressing C3 were effective in detecting cell death following drug treatment, including the detection of the tipping point of apoptosis. The drug-induced cell apoptosis was also observed in both zebrafish embryos and nude mice xenograft models. Overall, the FRET-based platform, through in vivo imaging, is potentially useful to improve drug screening efficiency.

apoptosis cancer therapy drug discovery FRET technique xenograft model

SCI ; EI

英语

通讯

Biotechnology & Applied Microbiology ; Science & Technology - Other Topics

Biotechnology & Applied Microbiology ; Multidisciplinary Sciences

WOS:000767126900001

FRONTIERS MEDIA SA

20221011745389

Cell culture ; Diseases ; Energy transfer ; Forster resonance energy transfer ; Mammals ; Oncology

Medicine and Pharmacology:461.6 ; Biology:461.9 ; Inorganic Compounds:804.2 ; Classical Physics; Quantum Theory; Relativity:931

Web of Science

1.Air Force Med Univ, Xijing Hosp, Dept Radiat Oncol, Xian, Peoples R China
2.Univ Oxford, Nuffield Dept Clin Neurosci, New Biochem Bldg, Oxford, England
3.Univ Oxford, Kavli Inst Nanosci Discovery, New Biochem Bldg, Oxford, England
4.George Washington Univ, Sch Med & Hlth Sci, Dept Biochem & Mol Med, Columbia, WA USA
5.Southern Univ Sci & Technol, Sch Life Sci, Dept Biol, Shenzhen, Peoples R China
6.Chinese Univ Hong Kong, Sch Life & Hlth Sci, Shenzhen, Peoples R China
7.Sun Yat Sen Univ, Affiliated Hosp 5, Guangdong Prov Key Lab Biomed Imaging, Zhuhai, Peoples R China
8.Sun Yat Sen Univ, Affiliated Hosp 5, Guangdong Prov Engn Res Ctr Mol Imaging, Zhuhai, Peoples R China

Huang, Shigao,Jiang, Cheng,Mughal, Muhammad Jameel,et al. An In Vivo Fluorescence Resonance Energy Transfer-Based Imaging Platform for Targeted Drug Discovery and Cancer Therapy[J]. Frontiers in Bioengineering and Biotechnology,2022,10.

Huang, Shigao,Jiang, Cheng,Mughal, Muhammad Jameel,Wang, Guanyu,&Xing, Fuqiang.(2022).An In Vivo Fluorescence Resonance Energy Transfer-Based Imaging Platform for Targeted Drug Discovery and Cancer Therapy.Frontiers in Bioengineering and Biotechnology,10.

Huang, Shigao,et al."An In Vivo Fluorescence Resonance Energy Transfer-Based Imaging Platform for Targeted Drug Discovery and Cancer Therapy".Frontiers in Bioengineering and Biotechnology 10(2022).