Cryo-EM structures reveal distinct apo conformations of sortilin-related receptor SORLA

Zhang，Xi1,2,3; Wu，Cang4; Song，Zhihong1; Sun，Dayong1; Zhai，Liting5; Liu，Chuang3

2022-04-16

10.1016/j.bbrc.2022.01.108

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   影响因子和分区

0006-291X

1090-2104

Sorting-related receptor with A-type repeats (SORLA) is an important receptor for regulating normal cellular functions via protein sorting. Here, we determined the structures of the full-length SORLA and identified two distinct conformations of apo-SORLA using single-particle cryogenic electron microscopy. In contrast to homologous proteins, both monomer and dimer forms of SORLA existed in a neutral solution. Only three hydrogen bonds in the vicinity of the dimer interface implied the involvement in dimerization. The orientation of residue R490 was a key point for ligand binding. These results suggest a unique mechanism of SORLA dimerization for protein trafficking.

Apo-SORLA Conformation change Cryo-EM structure Dimerization

SCI

英语

通讯

WOS:000759480800012

BIOLOGY & BIOCHEMISTRY

2-s2.0-85124830222

Scopus

1.Department of Gastroenterology,The First Affiliated Hospital of Shenzhen University,Shenzhen People's Second Hospital,Shenzhen,518000,China
2.Institute of Synthetic Biology,Shenzhen Institutes of Advanced Technology,Chinese Academy of Sciences,Shenzhen,518055,China
3.Cryo-EM Centre,Southern University of Science and Technology,Shenzhen,518055,China
4.Department of Biology,School of Life Sciences,Southern University of Science and Technology,Shenzhen,518055,China
5.ChEM-H/Neuroscience Research Complex 290 Jane Stanford Way,Stanford University,Stanford,94305,United States

Zhang，Xi,Wu，Cang,Song，Zhihong,et al. Cryo-EM structures reveal distinct apo conformations of sortilin-related receptor SORLA[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2022,600:75-79.

Zhang，Xi,Wu，Cang,Song，Zhihong,Sun，Dayong,Zhai，Liting,&Liu，Chuang.(2022).Cryo-EM structures reveal distinct apo conformations of sortilin-related receptor SORLA.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,600,75-79.

Zhang，Xi,et al."Cryo-EM structures reveal distinct apo conformations of sortilin-related receptor SORLA".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 600(2022):75-79.