Contrast-enhanced ultrasound imaging using long-circulating cationic magnetic microbubbles in vitro and in vivo validations

Liu，Yingying1; Lai，Xiaoshu1; Zhu，Yao1; Guo，Fengjuan1; Su，Lili1; Arkin，Gulzira1; He，Tianzhen1; Xu，Jinfeng1; Ran，Haitao2

2022-03-25

10.1016/j.ijpharm.2021.121299

INTERNATIONAL JOURNAL OF PHARMACEUTICS   影响因子和分区

0378-5173

1873-3476

Traditional encapsulated microbubbles are recently used as delivery carriers for drugs and genes, but they have low efficiency. If the local microbubble concentration could be increased, this might be able to improve the therapeutic efficacy of diseases. In this study, we developed novel cationic magnetic microbubbles (MB), which could simultaneously realize targeted aggregation under a magnetic field as well as ultrasonographic real-time visualization. Their physicochemical properties, biocompatibility, ultrasonography, magnetic response characteristics, and biodistribution were systematically evaluated. Here, the MB were 2.55 ± 0.14 µm in size with a positive zeta potential, and had a good biocompatibility. They were able to enhance ultrasonographic contrast both in vitro and in vivo. MB could be attracted by an external magnet for directional movement and aggregation in vitro. We confirmed that MB also had a great magnetic response in vivo, by means of fluorescence imaging and contrast-enhanced ultrasound imaging. Following intravenous injection into tumor-bearing mice, MB showed excellent stability in the internal circulation, and could accumulate in the tumor vasculature through magnetic targeting. With the excellent combination of magnetic response and acoustic properties, cationic magnetic microbubbles (MB) have promising potential for use as a new kind of drug/gene carrier for theranostics in the future.

Breast cancer Contrast-enhanced ultrasound imaging Magnetic targeting Microbubbles

SCI

英语

第一 ; 通讯

National Natural Science Foundation of China[81771841] ; Project of Innovation of the Science and Technology Commission of Shenzhen City[JCYJ20190807145609482] ; Discipline Construction Capacity Improvement Project of the Shenzhen Health and Family Planning Commission[SZXJ2018014] ; Guangdong Basic and Applied Basic Research Foundation[2019A1515111132]

Pharmacology & Pharmacy

Pharmacology & Pharmacy

WOS:000773658500006

ELSEVIER

PHARMACOLOGY & TOXICOLOGY

2-s2.0-85125369206

Scopus

1.Shenzhen Medical Ultrasound Engineering Center,Department of Ultrasonography,Shenzhen People's Hospital,The Second Clinical Medical College,Jinan University,The First Affiliated Hospital,Southern University of Science and Technology,Shenzhen,518020,China
2.Chongqing Key Laboratory of Ultrasound Molecular Imaging,The Second Affiliated Hospital of Chongqing Medical University,Chongqing,400010,China

Liu，Yingying,Lai，Xiaoshu,Zhu，Yao,et al. Contrast-enhanced ultrasound imaging using long-circulating cationic magnetic microbubbles in vitro and in vivo validations[J]. INTERNATIONAL JOURNAL OF PHARMACEUTICS,2022,616.

Liu，Yingying.,Lai，Xiaoshu.,Zhu，Yao.,Guo，Fengjuan.,Su，Lili.,...&Ran，Haitao.(2022).Contrast-enhanced ultrasound imaging using long-circulating cationic magnetic microbubbles in vitro and in vivo validations.INTERNATIONAL JOURNAL OF PHARMACEUTICS,616.

Liu，Yingying,et al."Contrast-enhanced ultrasound imaging using long-circulating cationic magnetic microbubbles in vitro and in vivo validations".INTERNATIONAL JOURNAL OF PHARMACEUTICS 616(2022).