Structure and nanomechanics of collagen fibrils in articular cartilage at different stages of osteoarthritis

Shao, Jundong1,2; Lin, Lijun3; Tang, Bin1; Du, Chang2

2014

10.1039/c4ra08997a

RSC Advances   影响因子和分区

2046-2069

2046-2069

This study aimed to investigate the variation of structure and nanomechanical properties of human articular cartilage (AC) at different stages of osteoarthritis (OA). The nanoscale morphology, chemical composition, variation of chemical structure, surface adhesion force and elastic modulus were examined. The thickening of collagen fibrils and a decreasing trend of their D-periodic banding patterns were observed along with the progression of OA. The calcium and phosphate concentration in AC showed an increasing trend from Grade 0 to Grade 3. These findings suggest that collagen fibrils have reduced intermolecular interactions, greater molecular disorder and decreased amount and stability of crosslinks in collagen, which may affect the stability of collagen fibrils. The surface adhesion force showed a decreasing trend while the elastic modulus of individual collagen fibrils showed an increasing trend with the advancement of OA, which was confirmed to have a direct functional consequence in the nanostructure of collagen fibrils. This study is helpful for understanding OA pathogenesis and providing an efficient strategy for pre-symptomatic diagnostics of OA.

SCI ; EI

英语

第一 ; 通讯

ShenZhen Government[201208303000415]

Chemistry

Chemistry, Multidisciplinary

WOS:000344387400014

ROYAL SOC CHEMISTRY

20144400148085

Adhesion ; Cartilage ; Crosslinking ; Elastic moduli ; Morphology

Biological Materials and Tissue Engineering:461.2 ; Chemical Reactions:802.2 ; Materials Science:951

Web of Science

1.South Univ Sci & Technol China, Dept Mat Sci & Engn, Shenzhen 518055, Peoples R China
2.South Univ Sci & Technol China, Sch Mat Sci & Engn, Guangzhou 510641, Guangdong, Peoples R China
3.Soouthern Med Univ, Zhujiang Hosp, Dept Orthoped, Guangzhou, Guangdong, Peoples R China

Shao, Jundong,Lin, Lijun,Tang, Bin,et al. Structure and nanomechanics of collagen fibrils in articular cartilage at different stages of osteoarthritis[J]. RSC Advances,2014,4(93):51165-51170.

Shao, Jundong,Lin, Lijun,Tang, Bin,&Du, Chang.(2014).Structure and nanomechanics of collagen fibrils in articular cartilage at different stages of osteoarthritis.RSC Advances,4(93),51165-51170.

Shao, Jundong,et al."Structure and nanomechanics of collagen fibrils in articular cartilage at different stages of osteoarthritis".RSC Advances 4.93(2014):51165-51170.