Accurate measurement of stiffness of leukemia cells and leukocytes using an optical trap by a rate-jump method

Zhou, Z. L.1; Hui, T. H.1; Tang, B.2; Ngan, A. H. W.1

2014

10.1039/c3ra45835k

RSC Advances   影响因子和分区

2046-2069

2046-2069

Accurate measurement of the elastic modulus of soft biological cells in the micro/nano scale range is still a challenging task. Tests involving constant-rate loading often yield results that are rate dependent, due to the viscous component of the deformation. In this work, a rate-jump indentation method was employed in an optical tweezers system to measure the stiffness of non-adherent blood cells, which are the softest types of cells. Compared to the traditional Hertzian method of indentation, the rate-jump method is found to be able to yield invariant elastic modulus from K562 myelogenous leukemia cells. The optical tweezers indentation method proposed can therefore serve as a standard protocol for obtaining the intrinsic elastic modulus of extremely soft cells, with applied forces in the pico-newton range. This method is also found to be effective in grading the stiffness values of myelogenous leukemia cell lines (K562 and HL60) and normal leukocytes, indicating that it can be used to identify normal cells from diseased counterparts without biochemical analysis.

SCI ; EI

英语

其他

Shenzhen Government, P.R. China[201208303000415]

Chemistry

Chemistry, Multidisciplinary

WOS:000330800300007

ROYAL SOC CHEMISTRY

20140717305834

Blood ; Cell culture ; Cells ; Diseases ; Elastic moduli ; Grading ; Optical tweezers ; Stiffness

Biological Materials and Tissue Engineering:461.2 ; Laser Applications:744.9 ; Materials Science:951

Web of Science

1.Univ Hong Kong, Dept Mech Engn, Hong Kong, Hong Kong, Peoples R China
2.South Univ Sci & Technol China, Dept Mat Sci & Engn, Shenzhen, Peoples R China

Zhou, Z. L.,Hui, T. H.,Tang, B.,et al. Accurate measurement of stiffness of leukemia cells and leukocytes using an optical trap by a rate-jump method[J]. RSC Advances,2014,4(17):8453-8460.

Zhou, Z. L.,Hui, T. H.,Tang, B.,&Ngan, A. H. W..(2014).Accurate measurement of stiffness of leukemia cells and leukocytes using an optical trap by a rate-jump method.RSC Advances,4(17),8453-8460.

Zhou, Z. L.,et al."Accurate measurement of stiffness of leukemia cells and leukocytes using an optical trap by a rate-jump method".RSC Advances 4.17(2014):8453-8460.