High resolution autofocus for spatial temporal biomedical research

Li, Sihong1; Cui, Xiaodong1; Huang, Wei1,2,3

2013-11

10.1063/1.4829616

REVIEW OF SCIENTIFIC INSTRUMENTS   影响因子和分区

0034-6748

1089-7623

Maintaining focus has been a critical but challenging issue in optical microscopy, particularly for microscopic imaging systems currently used in biomedical research. During live cell imaging, environmental temperature fluctuations and other factors contribute to the unavoidable focus drift. For single molecular imaging and super resolution, focus drift can be significant even over short durations. The current commercial and experimental solutions are either optically complicated, expensive, or with limited axial resolution. Here, we present a simple autofocus solution based on low cost solid state laser and imaging sensor. By improving the optical train design and using real-time data analysis, improvement in axial resolution by approximately two orders of magnitudes over the focal depth of microscope objectives can be achieved. This solution has been tested for prolonged live cell imaging for fast ramping up in environmental chamber temperature and large daily swing in room temperature. In addition, this system can be used to spatial-temporally measure the surface for three-dimensional cell culture and tissue engineering, with flexibility that exceeds commercially available systems. (C) 2013 AIP Publishing LLC.

SCI ; EI

英语

其他

University of Hong Kong seed[104000978] ; University of Hong Kong seed[104001876]

Instruments & Instrumentation ; Physics

Instruments & Instrumentation ; Physics, Applied

WOS:000329982000035

AMER INST PHYSICS

20134917056822

Cell culture ; Environmental chambers ; Molecular imaging ; Solid state lasers ; Tissue engineering

Biomedical Engineering:461.1 ; Solid State Lasers:744.4

CHEMISTRY

Web of Science

1.Univ Hong Kong, Dept Phys, Pokfulam, Hong Kong, Peoples R China
2.Univ Hong Kong, Dept Biochem, Pokfulam, Hong Kong, Peoples R China
3.South Univ Sci & Technol China, Shenzhen, Peoples R China

Li, Sihong,Cui, Xiaodong,Huang, Wei. High resolution autofocus for spatial temporal biomedical research[J]. REVIEW OF SCIENTIFIC INSTRUMENTS,2013,84(11).

Li, Sihong,Cui, Xiaodong,&Huang, Wei.(2013).High resolution autofocus for spatial temporal biomedical research.REVIEW OF SCIENTIFIC INSTRUMENTS,84(11).

Li, Sihong,et al."High resolution autofocus for spatial temporal biomedical research".REVIEW OF SCIENTIFIC INSTRUMENTS 84.11(2013).