AFM nanoindentation detection of the elastic modulus of tongue squamous carcinoma cells with different metastatic potentials

Zhou, Zhoulong2; Zheng, Chaoxu3; Li, Su4; Zhou, Xiaofeng5; Liu, Zhonghua1; He, Qianting1; Zhang, Ningning1; Ngan, Alfonso2; Tang, Bin2,6; Wang, Anxun1

2013-10

10.1016/j.nano.2013.04.001

Nanomedicine-Nanotechnology Biology and Medicine   影响因子和分区

1549-9634

1549-9642

Although significant advances have been made in understanding the molecular mechanisms that influence tongue squamous cell carcinoma (TSCC) metastasis, less is known about the association between the cellular elastic modulus and TSCC metastasis. Atomic force microscopy (AFM) nanoindentation via the rate-jump method was used to detect the elastic modulus of TSCC cells from patients and cell lines with different metastatic potentials. TSCC cells with higher metastatic potential showed decreases in the elastic modulus compared to TSCC cells with lower metastatic potential. Moreover, the decrease in elastic modulus was accompanied with epithelial-mesenchymal transition (EMT), cytoskeleton (F-actin and beta-tubulin) changes, small nucleus size and large nucleus/cytoplasm (N/C) ratio. The present findings demonstrate a close relationship between the cellular elastic modulus and the metastasis of TSCC. The elastic modulus detected by AFM nanoindentation via the rate-jump method can potentially be used to grade the metastatic potential of TSCC. (C) 2013 Elsevier Inc. All rights reserved.

Tongue squamous cell carcinoma Elastic modulus Metastasis Atomic force microscopy Nanoindentation

SCI ; EI

英语

其他

National Institutes of Health PHS grants[CA139596]

Science & Technology - Other Topics ; Research & Experimental Medicine

Nanoscience & Nanotechnology ; Medicine, Research & Experimental

WOS:000324520900004

ELSEVIER SCIENCE BV

20133916773723

Atomic force microscopy ; Cell culture ; Elastic moduli ; Medical nanotechnology ; Nanoindentation ; Pathology ; Proteins

Biological Materials and Tissue Engineering:461.2 ; Medicine and Pharmacology:461.6 ; Optical Devices and Systems:741.3 ; Nanotechnology:761 ; Organic Compounds:804.1 ; Materials Science:951

Web of Science

1.Sun Yat Sen Univ, Dept Oral & Maxillofacial Surg, Affiliated Hosp 1, Guangzhou 510275, Guangdong, Peoples R China
2.Univ Hong Kong, Dept Mech Engn, Hong Kong, Hong Kong, Peoples R China
3.Sun Yat Sen Univ, Dept Gen Surg, Affiliated Hosp 1, Guangzhou 510275, Guangdong, Peoples R China
4.Sun Yat Sen Univ, Tumor Hosp, Dept Med, Guangzhou 510275, Guangdong, Peoples R China
5.Univ Illinois, Coll Dent, Dept Periodont, Chicago, IL USA
6.South Univ Sci & Technol China, Dept Micro Nano Mat & Devices, Shengzhen, Peoples R China

Zhou, Zhoulong,Zheng, Chaoxu,Li, Su,et al. AFM nanoindentation detection of the elastic modulus of tongue squamous carcinoma cells with different metastatic potentials[J]. Nanomedicine-Nanotechnology Biology and Medicine,2013,9(7):864-874.

Zhou, Zhoulong.,Zheng, Chaoxu.,Li, Su.,Zhou, Xiaofeng.,Liu, Zhonghua.,...&Wang, Anxun.(2013).AFM nanoindentation detection of the elastic modulus of tongue squamous carcinoma cells with different metastatic potentials.Nanomedicine-Nanotechnology Biology and Medicine,9(7),864-874.

Zhou, Zhoulong,et al."AFM nanoindentation detection of the elastic modulus of tongue squamous carcinoma cells with different metastatic potentials".Nanomedicine-Nanotechnology Biology and Medicine 9.7(2013):864-874.