南方科技大学知识苑(SUSTech KC): HPV+ HNSCC-derived exosomal miR-9-5p inhibits TGF-beta signaling-mediated fibroblast phenotypic transformation through NOX4

题名	HPV+ HNSCC-derived exosomal miR-9-5p inhibits TGF-beta signaling-mediated fibroblast phenotypic transformation through NOX4
作者	Wang, Bozhi 1,2; Zhang, Siwei 1,2; Tong, Fangjia 3; Wang, Yan 1,2; Wei, Lanlan 1,2,3
通讯作者	Wei, Lanlan
发表日期	2022-03-01
DOI	10.1111/cas.15281
发表期刊	CANCER SCIENCE 影响因子和分区
ISSN	1347-9032
EISSN	1349-7006
摘要	Human papillomavirus (HPV) is a significant risk factor for head and neck squamous cell carcinoma (HNSCC). HPV+ HNSCC patients have a higher survival rate, which may be related to its unique tumor microenvironment. Exosomes are emerging as a communication tool between tumor cells and the tumor microenvironment, including cancer-associated fibroblasts (CAFs). In this study, 111 clinical samples tissues and public sequencing data were analyzed. Our study found fewer CAFs infiltrated in HPV+ HNSCC, and poor CAF infiltration level was associated with a good prognosis. HPV+ HNSCC cell-derived exosomes can significantly reduce the phenotypic transformation of fibroblasts. miR-9-5p, as a miRNA enriched in HPV+ HNSCC cell-derived exosomes, can be transferred to fibroblasts. miR-9-5p mimic transfection decreased the expression of NOX4 and the level of intracellular reactive oxygen species (ROS), which inhibited the transforming growth factor beta 1(TGF-beta 1)-induced increase of alpha SMA levels. Therefore, these results indicated that HPV+ HNSCC-derived exosomal miR-9-5p inhibits TGF-beta signaling-mediated fibroblast phenotypic transformation through NOX4, which is related to the excellent prognosis of HPV patients.
关键词	cancer-associated fibroblasts exosome head and neck squamous cell carcinoma HPV miR-9-5p
相关链接	[来源记录]
收录类别	SCI
语种	英语
学校署名	通讯
资助项目	National Natural Science Foundation of China[82102822] ; Shenzhen Longgang Fund for Science and Technology Project[LGKCYLWS2020101] ; Shenzhen Science and Technology RD Fund[JCYJ20210324131607019]
WOS研究方向	Oncology
WOS类目	Oncology
WOS记录号	WOS:000763424600001
出版者	WILEY
ESI学科分类	CLINICAL MEDICINE
来源库	Web of Science
引用统计	被引频次[WOS]：19
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/308543
专题	南方科技大学第二附属医院
作者单位	1.Harbin Med Univ, Dept Microbiol, Harbin, Peoples R China 2.Harbin Med Univ, Wu Lien Teh Inst, Harbin, Peoples R China 3.Southern Univ Sci & Technol, Peoples Hosp Shenzhen 3, Natl Clin Res Ctr Infect Dis, Inst Hepatol,Hosp 2, Shenzhen, Peoples R China
通讯作者单位	南方科技大学第二附属医院
推荐引用方式 GB/T 7714	Wang, Bozhi,Zhang, Siwei,Tong, Fangjia,et al. HPV+ HNSCC-derived exosomal miR-9-5p inhibits TGF-beta signaling-mediated fibroblast phenotypic transformation through NOX4[J]. CANCER SCIENCE,2022.
APA	Wang, Bozhi,Zhang, Siwei,Tong, Fangjia,Wang, Yan,&Wei, Lanlan.(2022).HPV+ HNSCC-derived exosomal miR-9-5p inhibits TGF-beta signaling-mediated fibroblast phenotypic transformation through NOX4.CANCER SCIENCE.
MLA	Wang, Bozhi,et al."HPV+ HNSCC-derived exosomal miR-9-5p inhibits TGF-beta signaling-mediated fibroblast phenotypic transformation through NOX4".CANCER SCIENCE (2022).