Ex vivo cartilage explant model for the evaluation of chondrocyte-targeted exosomes

Kan, Ouyang1,2; Xu, Meiquan3; Liang, Yujie4; Xu, Xiao2; Xu, Limei2; Wen, Caining1,2; Qin, Zhuan2; Xie, Yixin2; Zhang, Huawei5; Duan, Li2; Wang, Daping1,2,5

2022

10.32604/biocell.2022.018788

BIOCELL   影响因子和分区

0327-9545

1667-5746

There is no efficient tracking system available for the therapeutic molecules delivered to cartilage. The dense matrix covering the cartilage surface is the main biological barrier that the therapeutic molecules must overcome. In this study, we aimed to establish a system that can dynamically and effectively track the therapeutic molecules delivered to cartilage. To this aim, we adopted bovine and human cartilage explants as ex vivo models for chondrocyte-targeted exosome dispersion. The efficiency of drug delivery was evaluated using frozen sections. The results of this study showed that the penetration and distribution of chondrocyte-targeted exosomes in cartilage explants can be tracked dynamically. Thus, ex vivo cartilage explants provide an effective and economic system to evaluate therapeutic drugs encapsulated in chondrocyte-targeted exosomes in preclinical studies.

Cartilage explants Exosomes Targeted delivery

SCI

英语

通讯

National Natural Science Foundation of China[81972116,81972085,81772394,31900046] ; Key Program of Natural Science Foundation of Guangdong Province[2018B0303110003] ; Guangdong International Cooperation Project[2021A0505030011] ; Shenzhen Science and Technology Projects["GJHZ20200731095606019","JCYJ20170817172023838","JCYJ20170306092215436","JCYJ20170413161649437"] ; China Postdoctoral Science Foundation[2020M682907]

Life Sciences & Biomedicine - Other Topics

Biology

WOS:000762293000019

TECH SCIENCE PRESS

BIOLOGY & BIOCHEMISTRY

Web of Science

1.Guangzhou Med Univ, Guangzhou 510095, Peoples R China
2.Shenzhen Univ, Affiliated Hosp 1, Shenzhen Peoples Hosp 2, Dept Sports Med,Guangdong Prov Res Ctr Artificial, Shenzhen 518035, Peoples R China
3.Shenzhen Univ, Affiliated Hosp 1, Shenzhen Peoples Hosp 2, Dept Pathol, Shenzhen 518035, Peoples R China
4.Shenzhen Mental Hlth Ctr, Shenzhen Kangning Hosp, Shenzhen Inst Mental Hlth, Shenzhen Key Lab Psychol Healthcare, Shenzhen 518020, Peoples R China
5.Southern Univ Sci & Technol, Dept Biomed Engn, Shenzhen 518055, Peoples R China

Kan, Ouyang,Xu, Meiquan,Liang, Yujie,et al. Ex vivo cartilage explant model for the evaluation of chondrocyte-targeted exosomes[J]. BIOCELL,2022,46(6).

Kan, Ouyang.,Xu, Meiquan.,Liang, Yujie.,Xu, Xiao.,Xu, Limei.,...&Wang, Daping.(2022).Ex vivo cartilage explant model for the evaluation of chondrocyte-targeted exosomes.BIOCELL,46(6).

Kan, Ouyang,et al."Ex vivo cartilage explant model for the evaluation of chondrocyte-targeted exosomes".BIOCELL 46.6(2022).