南方科技大学知识苑(SUSTech KC): Safeguard DCL2-Dependent 22-nt siRNA generation by DCL1

题名	Safeguard DCL2-Dependent 22-nt siRNA generation by DCL1
作者	Lu，Dongdong1,2,3 ; Feng，Li2,3 ; Zhai，Jixian3 ; Li，Bosheng2,3 ; Xi，Mengli 1
通讯作者	Li，Bosheng; Xi，Mengli
发表日期	2022-05-21
DOI	10.1016/j.bbrc.2022.03.076
发表期刊	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 影响因子和分区
ISSN	0006-291X
EISSN	1090-2104
卷号	605页码:97-103
摘要	MicroRNAs (miRNAs) and small interfering RNAs (siRNAs) are crucial for plant growth and development via mediating post-transcriptional gene silencing. In wild-type Arabidopsis, DICER-LIKE 2 (DCL2)-dependent 22-nt siRNAs are rare, whereas DCL1 and DCL4-dependent 21-nt miRNAs and siRNAs are highly abundant. DCL4 naturally inhibits DCL2 in producing abundant 22-nt siRNAs from endogenous transcripts, but whether DCL1 suppresses endogenous 22-nt siRNA production and the extent of repression are still unknown. Here, we report that DCL1 and DCL2 cleaved both miRNA precursors and coding transcript-derived double-stranded RNAs. In a dcl1 dcl4 double mutant, massive 22-nt siRNAs were produced from endogenous protein-coding genes (genic siRNAs). Compared with wild-type, the 22-nt genic siRNAs derived from the Nitrate Reductase 1 (NIA1), NIA2, DIACYLGLYCEROL ACYLTRANSFERASES 3 (DGAT3), SUPPRESSOR OF MAX2 1-LIKE 5 (SMXL5), and SMXL4 in dcl1 dcl4 increased up to 95%. Our analysis further indicated that the 22-nt genic siRNAs in dcl1 dcl4 were mainly loaded into ARGONAUTE 1 (AGO1) or AGO2. Thus, our results demonstrated that both DCL1 and DCL4 safeguard post-transcriptional gene silencing, preventing the production of DCL2-dependent 22-nt genic siRNAs from disrupting plant growth and development.
关键词	Arabidopsis ARGONAUTE DICER miRNAs siRNAs
相关链接	[Scopus记录]
收录类别	SCI
语种	英语
学校署名	通讯
资助项目	Program for Guangdong Introducing Innovative and Entrepreneurial Teams[2016ZT06S172]
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics
WOS类目	Biochemistry & Molecular Biology ; Biophysics
WOS记录号	WOS:000791299100001
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
ESI学科分类	BIOLOGY & BIOCHEMISTRY
Scopus记录号	2-s2.0-85126561292
来源库	Scopus
引用统计	被引频次[WOS]：0
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/313156
专题	南方科技大学-北京大学植物与食品联合研究所生命科学学院生命科学学院_生物系
作者单位	1.Key Laboratory of Forest Genetics and Biotechnology of Ministry of Education,Nanjing Forestry University,Nanjing,210037,China 2.Institute of Advanced Agricultural Sciences,Peking University,Weifang,261325,China 3.Institute of Plant and Food Science,Department of Biology,Southern University of Science and Technology,Shenzhen,518055,China
第一作者单位	南方科技大学-北京大学植物与食品联合研究所; 生物系; 生命科学学院
通讯作者单位	南方科技大学-北京大学植物与食品联合研究所; 生物系; 生命科学学院
推荐引用方式 GB/T 7714	Lu，Dongdong,Feng，Li,Zhai，Jixian,et al. Safeguard DCL2-Dependent 22-nt siRNA generation by DCL1[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2022,605:97-103.
APA	Lu，Dongdong,Feng，Li,Zhai，Jixian,Li，Bosheng,&Xi，Mengli.(2022).Safeguard DCL2-Dependent 22-nt siRNA generation by DCL1.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,605,97-103.
MLA	Lu，Dongdong,et al."Safeguard DCL2-Dependent 22-nt siRNA generation by DCL1".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 605(2022):97-103.