Metagenomic Analysis Reveals Neisseria bacilliformis Variation in the Early Childhood Caries Plaque Microbiome

Xiao, Xiaofen1; He, Shandan1; He, Fei1; Wu, Xiaoyun2; Zheng, Yuyan1

2021-10-21

10.1155/2021/2774772

Evidence-based Complementary and Alternative Medicine   影响因子和分区

1741-427X

1741-4288

The progression of early childhood caries (ECC) is caused by microbial colonized in dental plaque. However, the association framework both from 16s genus down to high resolution metagenomic strain level and from composition to genome function analysis on caries lacks. 16S rRNA sequence revealed the composition of 3-6 years dental caries (ECC, n = 29), and severe dental caries (SECC, n = 36) children are significantly different from caries-free controls (CF, n = 31). Especially, genus Neisseria is enriched in caries (P < 0.05). Metagenomics sequence of 3 ECCs, 3 SECCs, and 3 CFs reveals Neisseria bacilliformis ATCC BAA-1200 in genus Neisseria is also significantly enriched in caries (P < 0.05). Then, we recovered high-quality metagenomic assembly genomes (MAG), named bin 86, which have 99% identity with Neisseria bacilliformis ATCC BAA-1200 genome. Function analysis of Neisseria bacilliformis ATCC BAA-1200 genome shows its metabolism power of sugar and adhesion, colonization, acid production, and acid tolerance ability, which suggested Neisseria bacilliformis ATCC BAA-1200 may serve as a biomarker for childhood caries.

SCI

英语

第一 ; 通讯

Integrative & Complementary Medicine

Integrative & Complementary Medicine

WOS:000769395300001

HINDAWI LTD

Web of Science

1.Southern Univ Sci & Technol, Affiliated Hosp 1, Jinan Univ, Clin Med Sch 2,Shenzhen Peoples Hosp,Dept Stomato, Shenzhen 518020, Guangdong, Peoples R China
2.Southern Univ Sci & Technol, Affiliated Hosp 1, Jinan Univ, Clin Med Sch 2,Shenzhen Peoples Hosp, Shenzhen 518020, Guangdong, Peoples R China

Xiao, Xiaofen,He, Shandan,He, Fei,et al. Metagenomic Analysis Reveals Neisseria bacilliformis Variation in the Early Childhood Caries Plaque Microbiome[J]. Evidence-based Complementary and Alternative Medicine,2021,2021.

Xiao, Xiaofen,He, Shandan,He, Fei,Wu, Xiaoyun,&Zheng, Yuyan.(2021).Metagenomic Analysis Reveals Neisseria bacilliformis Variation in the Early Childhood Caries Plaque Microbiome.Evidence-based Complementary and Alternative Medicine,2021.

Xiao, Xiaofen,et al."Metagenomic Analysis Reveals Neisseria bacilliformis Variation in the Early Childhood Caries Plaque Microbiome".Evidence-based Complementary and Alternative Medicine 2021(2021).