南方科技大学知识苑(SUSTech KC): Multiplexed Lab-on-a-Chip Bioassays for Testing Antibodies against SARS-CoV-2 and Its Variants in Multiple Individuals

题名	Multiplexed Lab-on-a-Chip Bioassays for Testing Antibodies against SARS-CoV-2 and Its Variants in Multiple Individuals
作者	Mou, Lei 1,2; Zhang, Yingying 1,3; Feng, Yao 1; Hong, Honghai 1; Xia, Yong 1; Jiang, Xingyu1,2
通讯作者	Xia, Yong; Jiang, Xingyu
发表日期	2022-02-08
DOI	10.1021/acs.analchem.1c04383
发表期刊	ANALYTICAL CHEMISTRY 影响因子和分区
ISSN	0003-2700
EISSN	1520-6882
卷号	94 期号:5页码:2510-2516
摘要	Neutralization assays that can measure neutralizing antibodies in serum are vital for large-scale serodiagnosis and vaccine evaluation. Here, we establish multiplexed lab-on-a-chip bioassays for testing antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its variants. Compared with enzyme-linked immunosorbent assay (ELISA), our method exhibits a low consumption of sample and reagents (10 mu L), a low limit of detection (LOD: 0.08 ng/mL), a quick sample-to-answer time (about 70 min), and multiplexed ability (5 targets in each of 7 samples in one assay). We can also increase the throughput as needed. The concentrations of antibodies against RBD, D614G, N501Y, E484K, and L452R/E484Q-mutants after two doses of vaccines are 6.6 +/- 3.6, 8.7 +/- 4.6, 3.4 +/- 2.8, 3.8 +/- 2.8, and 2.8 +/- 2.3 ng/mL, respectively. This suggests that neutralizing activities against N501Y, E484K, and L452R/E484Q-mutants were less effective than RBD and D614G-mutant. We performed a plaque reduction neutralization test (PRNT) for all volunteers. Compared with PRNT, our assay is fast, accurate, inexpensive, and multiplexed with multiple-sample processing ability, which is good for large-scale serodiagnosis and vaccine evaluation.
相关链接	[来源记录]
收录类别	SCI ; EI
语种	英语
重要成果	NI论文
学校署名	通讯
资助项目	National Key R&D Program of China["2018YFA0902600","2017YFA0205901"] ; National Natural Science Foundation of China[21535001,81730051,21761142006] ; Department of Education of Guangdong Province[2020KZDZX1169] ; Chinese Academy of Sciences["QYZDJ-SSW-SLH039","121D11KYSB20170026","XDA16020902"] ; Guangdong Innovative and Entrepreneurial Research Team Program[2019ZT08Y191] ; Guangzhou Municipal Science and Technology Bureau[202002020085] ; Shenzhen Bay Laboratory[SZBL2019062801004]
WOS研究方向	Chemistry
WOS类目	Chemistry, Analytical
WOS记录号	WOS:000763582800019
出版者	AMER CHEMICAL SOC
EI入藏号	20220711620114
EI主题词	Antibodies ; Diseases ; Lab-on-a-chip
EI分类号	Medicine and Pharmacology:461.6 ; Immunology:461.9.1 ; Semiconductor Devices and Integrated Circuits:714.2
ESI学科分类	CHEMISTRY
来源库	Web of Science
引用统计	被引频次[WOS]：8
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/313180
专题	工学院_生物医学工程系
作者单位	1.Guangzhou Med Univ, Dept Clin Lab, Affiliated Hosp 3, Guangzhou 510150, Guangdong, Peoples R China 2.Southern Univ Sci & Technol, Dept Biomed Engn, Shenzhen 518055, Guangdong, Peoples R China 3.Baoan Authent TCM Therapy Hosp, Dept Clin Lab, Shenzhen 518101, Guangdong, Peoples R China
第一作者单位	生物医学工程系
通讯作者单位	生物医学工程系
推荐引用方式 GB/T 7714	Mou, Lei,Zhang, Yingying,Feng, Yao,et al. Multiplexed Lab-on-a-Chip Bioassays for Testing Antibodies against SARS-CoV-2 and Its Variants in Multiple Individuals[J]. ANALYTICAL CHEMISTRY,2022,94(5):2510-2516.
APA	Mou, Lei,Zhang, Yingying,Feng, Yao,Hong, Honghai,Xia, Yong,&Jiang, Xingyu.(2022).Multiplexed Lab-on-a-Chip Bioassays for Testing Antibodies against SARS-CoV-2 and Its Variants in Multiple Individuals.ANALYTICAL CHEMISTRY,94(5),2510-2516.
MLA	Mou, Lei,et al."Multiplexed Lab-on-a-Chip Bioassays for Testing Antibodies against SARS-CoV-2 and Its Variants in Multiple Individuals".ANALYTICAL CHEMISTRY 94.5(2022):2510-2516.