Integrative analysis reveals a lineage-specific circular RNA landscape for adipo-osteogenesis of human mesenchymal stem cells

Huang, Hai-Bo1,2,3,4,6; Luo, Hai-Tao1,2,3,4; Wei, Na-Na1,2,3,7; Liu, Miao-Ling1,2,3; He, Fei1,2,3; Yang, Wei1,2,3; Dong, Jun4,5; Yang, Xiao-Fei1,2,3; Li, Fu-Rong1,2,3,4

2022-03-12

10.1186/s13287-022-02792-5

STEM CELL RESEARCH & THERAPY   影响因子和分区

1757-6512

["Background: The balance between osteogenesis and adipogenesis of mesenchymal stem cells (MSCs) is critical to skeletal development and diseases. As a research hotspot, circular RNAs (circRNAs) have expanded our understanding of a hidden layer of the transcriptome. Yet, their roles during adipo-osteogenesis remain poorly described.","Methods: The identity of human MSCs derived from bone marrow and adipose were first determined by flow cytometry, cellular staining, and quantitative polymerase chain reaction (qPCR). Multi-strategic RNA-sequencing was performed using Poly A, RiboMinus and RiboMinus/RNase R methods. Integrative analysis was performed to identify lineage-specific expressed circRNAs. The structural and expressional characteristics were identified by Sanger sequencing and qPCR, respectively. The regulatory effects of adipogenesis-specific circ-CRLF1 were confirmed using siRNA transcfection and qPCR.","Results: We generated a whole transcriptome map during adipo-osteogenesis based on 10 Poly A, 20 RiboMinus and 20 RiboMinus/RNase R datasets. A total of 31,326 circRNAs were identified and quantified from similar to 3.4 billion paired-end reads. Furthermore, the integrative analysis revealed that 1166 circRNA genes exhibited strong lineage-specific expression patterns. Their host genes were enriched in distinct biological functions, such as cell adhesion, cytokine signaling, and cell division. We randomly selected and validated the back-spliced junction sites and expression patterns of 12 lineage-specific circRNAs. Functional analysis indicated that circ-CRLF1 negatively regulated adipogenesis.","Conclusions: Our integrative analysis reveals an accurate and generally applicable lineage-specific circRNA landscape for adipo-osteogenesis of MSCs and provides a potential therapeutic target, circ-CRLF1, for the treatment of skeleton-related disease."]

Mesenchymal stem cells Osteogenesis Adipogenesis Circular RNAs CRLF1

SCI

英语

通讯

Cell Biology ; Research & Experimental Medicine

Cell & Tissue Engineering ; Cell Biology ; Medicine, Research & Experimental

WOS:000767936000003

BMC

Web of Science

1.Jinan Univ, Clin Med Coll 2, Shenzhen Peoples Hosp, Translat Med Collaborat Innovat Ctr, Shenzhen, Guangdong, Peoples R China
2.Southern Univ Sci & Technol, Affiliated Hosp 1, Shenzhen 518020, Guangdong, Peoples R China
3.Shenzhen Immune Cell Therapy Publ Serv Platform, Shenzhen Key Lab Stem Cell Res & Clin Transformat, Guangdong Engn Technol Res Ctr Stem Cell & Cell T, Shenzhen 518020, Guangdong, Peoples R China
4.Jinan Univ, Integrated Chinese & Western Med Postdoctoral Res, Guangzhou 510632, Guangdong, Peoples R China
5.Jinan Univ, State Adm Tradit Chinese Med, Key Lab, Dept Pathophysiol, Guangzhou 510632, Guangdong, Peoples R China
6.Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, Shenzhen 518124, Peoples R China
7.Kunpeng Inst Modern Agr Foshan, Foshan 528200, Peoples R China

Huang, Hai-Bo,Luo, Hai-Tao,Wei, Na-Na,et al. Integrative analysis reveals a lineage-specific circular RNA landscape for adipo-osteogenesis of human mesenchymal stem cells[J]. STEM CELL RESEARCH & THERAPY,2022,13(1).

Huang, Hai-Bo.,Luo, Hai-Tao.,Wei, Na-Na.,Liu, Miao-Ling.,He, Fei.,...&Li, Fu-Rong.(2022).Integrative analysis reveals a lineage-specific circular RNA landscape for adipo-osteogenesis of human mesenchymal stem cells.STEM CELL RESEARCH & THERAPY,13(1).

Huang, Hai-Bo,et al."Integrative analysis reveals a lineage-specific circular RNA landscape for adipo-osteogenesis of human mesenchymal stem cells".STEM CELL RESEARCH & THERAPY 13.1(2022).