Optical Clearing Agents for Optical Imaging Through Cartilage Tympanoplasties: A Preclinical Feasibility Study

Wang，Junzhe1; Chawdhary，Gaurav2; Yang，Xiaojie1,3; Morin，Francis4; Khalid-Raja，Mamoona5; Farrell，Joshua1; MacDougall，Dan1; Chen，Fangyi3; Morris，David P.6; Adamson，Robert B.A.1,6,7

2022-04-01

10.1097/MAO.0000000000003502

OTOLOGY & NEUROTOLOGY   影响因子和分区

1531-7129

1537-4505

HYPOTHESIS: Optical clearing agents (OCAs) can render cartilage tympanoplasty grafts sufficiently transparent to permit visualization of middle ear structures in an operated ear using optical coherence tomography (OCT) imaging. METHODS: Pieces of human tragal cartilage were treated with glycerol, a commonly used OCA. A reference reflector was imaged with OCT through the tympanoplasty as it cleared and the optical attenuation of the graft was measured. The reversibility of clearing and the dimensional changes associated with glycerol absorption were also measured. In a separate experiment, a human cadaveric temporal bone was prepared to simulate an ossiculoplasty surgery with cartilage replacement of the tympanic membrane. A partial ossicular replacement prosthesis (PORP) inserted in the ear was imaged with OCT through a 0.4mm cartilage graft optically cleared with glycerol. MAIN OUTCOME MEASURE: The optical attenuation of 0.4mm cartilage grafts decreased at 2.3+/-1.1 dB/min following treatment with glycerol, reaching a total decrease in attenuation of 13.6+/-5.9 dB after 7 minutes. The optical and dimensional effects of glycerol absorption were reversable following saline washout. In the temporal bone preparation, treatment of a cartilage graft with glycerol resulted in a 13 dB increase in signal-to-noise ratio and a 13 dB increase in contrast for visualizing the PORP through the graft with OCT. CONCLUSIONS: Optical clearing agents offer a potential pathway towards optical coherence tomography imaging of the middle ear in post-surgical ears with cartilage grafts.

Cartilage tympanoplasty Middle ear Non-invasive visualization Optical clearing agents Optical coherence tomography Prosthesis migration

SCI

英语

其他

MITACS[IT14716]

Neurosciences & Neurology ; Otorhinolaryngology

Clinical Neurology ; Otorhinolaryngology

WOS:000780251700019

LIPPINCOTT WILLIAMS & WILKINS

CLINICAL MEDICINE

2-s2.0-85126490712

Scopus

1.School of Biomedical Engineering,Dalhousie University,
2.Department of Otolaryngology,Royal Hallamshire Hospital,Sheffield,United Kingdom
3.Department of Biomedical Engineering,Southern University of Science and Technology,Shenzhen,China
4.Family Medicine,McGill University,Montreal,France
5.Stockport NHS Foundation Trust,Great Manchester,Stepping Hill Hospital,United Kingdom
6.Division of Otolaryngology Head & Neck Surgery,Department of Surgery,
7.Electrical and Computer Engineering Department,Dalhousie University,Halifax,Canada

Wang，Junzhe,Chawdhary，Gaurav,Yang，Xiaojie,et al. Optical Clearing Agents for Optical Imaging Through Cartilage Tympanoplasties: A Preclinical Feasibility Study[J]. OTOLOGY & NEUROTOLOGY,2022,43(4):e467-e474.

Wang，Junzhe.,Chawdhary，Gaurav.,Yang，Xiaojie.,Morin，Francis.,Khalid-Raja，Mamoona.,...&Adamson，Robert B.A..(2022).Optical Clearing Agents for Optical Imaging Through Cartilage Tympanoplasties: A Preclinical Feasibility Study.OTOLOGY & NEUROTOLOGY,43(4),e467-e474.

Wang，Junzhe,et al."Optical Clearing Agents for Optical Imaging Through Cartilage Tympanoplasties: A Preclinical Feasibility Study".OTOLOGY & NEUROTOLOGY 43.4(2022):e467-e474.