南方科技大学知识苑(SUSTech KC): The coupling mechanism of mammalian mitochondrial complex I

题名	The coupling mechanism of mammalian mitochondrial complex I
作者	Gu，Jinke 1,2; Liu，Tianya 1; Guo，Runyu 1; Zhang，Laixing 1; Yang，Maojun 1,3
通讯作者	Yang，Maojun
发表日期	2022-02-01
DOI	10.1038/s41594-022-00722-w
发表期刊	Nature Structural and Molecular Biology 影响因子和分区
ISSN	1545-9993
EISSN	1545-9985
卷号	29 期号:2页码:172-182
摘要	Mammalian respiratory complex I (CI) is a 45-subunit, redox-driven proton pump that generates an electrochemical gradient across the mitochondrial inner membrane to power ATP synthesis in mitochondria. In the present study, we report cryo-electron microscopy structures of CI from Sus scrofa in six treatment conditions at a resolution of 2.4–3.5 Å, in which CI structures of each condition can be classified into two biochemical classes (active or deactive), with a notably higher proportion of active CI particles. These structures illuminate how hydrophobic ubiquinone-10 (Q10) with its long isoprenoid tail is bound and reduced in a narrow Q chamber comprising four different Q10-binding sites. Structural comparisons of active CI structures from our decylubiquinone–NADH and rotenone–NADH datasets reveal that Q10 reduction at site 1 is not coupled to proton pumping in the membrane arm, which might instead be coupled to Q10 oxidation at site 2. Our data overturn the widely accepted previous proposal about the coupling mechanism of CI.
相关链接	[Scopus记录]
收录类别	SCI
语种	英语
重要成果	ESI高被引 ; NI论文
学校署名	通讯
WOS记录号	WOS:000753738000002
ESI学科分类	BIOLOGY & BIOCHEMISTRY
Scopus记录号	2-s2.0-85124481943
来源库	Scopus
引用统计	被引频次[WOS]：47
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/327759
专题	冷冻电镜中心
作者单位	1.Ministry of Education Key Laboratory of Protein Science,Beijing Advanced Innovation Center for Structural Biology,Beijing Frontier Research Center for Biological Structure,School of Life Sciences,Tsinghua University,Tsinghua-Peking Center for Life Sciences,Beijing,China 2.Department of Biochemistry and Molecular Biology,School of Basic Medical Sciences,Shenzhen University Health Science Center,Shenzhen,China 3.SUSTech Cryo-EM Facility Center,Southern University of Science & Technology,Shenzhen,China
通讯作者单位	冷冻电镜中心
推荐引用方式 GB/T 7714	Gu，Jinke,Liu，Tianya,Guo，Runyu,et al. The coupling mechanism of mammalian mitochondrial complex I[J]. Nature Structural and Molecular Biology,2022,29(2):172-182.
APA	Gu，Jinke,Liu，Tianya,Guo，Runyu,Zhang，Laixing,&Yang，Maojun.(2022).The coupling mechanism of mammalian mitochondrial complex I.Nature Structural and Molecular Biology,29(2),172-182.
MLA	Gu，Jinke,et al."The coupling mechanism of mammalian mitochondrial complex I".Nature Structural and Molecular Biology 29.2(2022):172-182.