南方科技大学知识苑(SUSTech KC): Molecular basis of differential receptor usage for naturally occurring CD55-binding and -nonbinding coxsackievirus B3 strains

题名	Molecular basis of differential receptor usage for naturally occurring CD55-binding and -nonbinding coxsackievirus B3 strains
作者	Wang，Qingling 1,2,3; Yang，Qian 4; Liu，Congcong 5,6; Wang，Guoqing 7; Song，Hao 8; Shang，Guijun 9; Peng，Ruchao 2; Qu，Xiao 2; Liu，Sheng 2; Cui，Yingzi 2; Wang，Peiyi5 ; Xu，Wenbo 4; Zhao，Xin 2; Qi，Jianxun 2; Yang，Mengsu 1; Gao，George F.1,2
通讯作者	Gao，George F.
发表日期	2022-01-25
DOI	10.1073/pnas.2118590119
发表期刊	PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 影响因子和分区
ISSN	0027-8424
EISSN	1091-6490
卷号	119 期号:4
摘要	Receptor usage defines cell tropism and contributes to cell entry and infection. Coxsackievirus B (CVB) engages coxsackievirus and adenovirus receptor (CAR), and selectively utilizes the decayaccelerating factor (DAF; CD55) to infect cells. However, the differential receptor usage mechanism for CVB remains elusive. This study identified VP3-234 residues (234Q/N/V/D/E) as critical population selection determinants during CVB3 virus evolution, contributing to diverse binding affinities to CD55. Cryoelectron microscopy (cryo-EM) structures of CD55-binding/nonbinding isolates and their complexes with CD55 or CAR were obtained under both neutral and acidic conditions, and the molecular mechanism of VP3-234 residues determining CD55 affinity/specificity for naturally occurring CVB3 strains was elucidated. Structural and biochemical studies in vitro revealed the dynamic entry process of CVB3 and the function of the uncoating receptor CAR with different pH preferences. This work provides detailed insight into the molecular mechanism of CVB infection and contributes to an in-depth understanding of enterovirus attachment receptor usage.
关键词	Attachment Or Uncoating Cryo-em Differential Receptor Usage Enteroviruses Entry
相关链接	[Scopus记录]
收录类别	SCI
语种	英语
重要成果	NI论文
学校署名	其他
WOS记录号	WOS:000758465600011
ESI学科分类	BIOLOGY & BIOCHEMISTRY;CLINICAL MEDICINE;MULTIDISCIPLINARY;PLANT & ANIMAL SCIENCE;ENVIRONMENT/ECOLOGY;SOCIAL SCIENCES, GENERAL;MICROBIOLOGY;ECONOMICS BUSINESS;IMMUNOLOGY;MATERIALS SCIENCE;MATHEMATICS;SPACE SCIENCE;MOLECULAR BIOLOGY & GENETICS;PHARMACOLOGY & TOXICOLOGY;CHEMISTRY;PSYCHIATRY/PSYCHOLOGY;NEUROSCIENCE & BEHAVIOR;PHYSICS;GEOSCIENCES;AGRICULTURAL SCIENCES;ENGINEERING
Scopus记录号	2-s2.0-85123091452
来源库	Scopus
引用统计	被引频次[WOS]：5
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/327790
专题	生命科学学院_生物系生命科学学院冷冻电镜中心
作者单位	1.Department of Biomedical Sciences,City University of Hong Kong,Hong Kong,Hong Kong 2.CAS Key Laboratory of Pathogen Microbiology and Immunology,Institute of Microbiology,Chinese Academy of Sciences (CAS),Beijing,100101,China 3.Shaanxi Natural Carbohydrate Resource Engineering Research Center,College of Food Science and Technology,Northwest University,Xi'an,710069,China 4.World Health Organization Western Pacific Region (WHO WPRO) Regional Polio Reference Laboratory and National Health Commission Key Laboratory for Medical Virology,National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing,102206,China 5.Cryo-EM Centre,Department of Biology,Southern University of Science and Technology,Shenzhen,518055,China 6.Institute for Hepatology,National Clinical Research Center for Infectious Disease,Shenzhen Third People's Hospital,Shenzhen,518112,China 7.Department of Pathogenobiology,The Key Laboratory of Zoonosis,Chinese Ministry of Education,College of Basic Medical Science,Jilin University,Changchun,130021,China 8.Research Network of Immunity and Health,Beijing Institutes of Life Science,Chinese Academy of Sciences,Beijing,100101,China 9.Shanxi Academy of Advanced Research and Innovation,Taiyuan,030032,China
推荐引用方式 GB/T 7714	Wang，Qingling,Yang，Qian,Liu，Congcong,et al. Molecular basis of differential receptor usage for naturally occurring CD55-binding and -nonbinding coxsackievirus B3 strains[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2022,119(4).
APA	Wang，Qingling.,Yang，Qian.,Liu，Congcong.,Wang，Guoqing.,Song，Hao.,...&Gao，George F..(2022).Molecular basis of differential receptor usage for naturally occurring CD55-binding and -nonbinding coxsackievirus B3 strains.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,119(4).
MLA	Wang，Qingling,et al."Molecular basis of differential receptor usage for naturally occurring CD55-binding and -nonbinding coxsackievirus B3 strains".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 119.4(2022).

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