Quantifying Bacterial Surface Swarming Motility on Inducer Gradient Plates

Guo，Siyu1; Liu，Zhao1; Yang，Yongshuai1; Chen，Jun2; Ho，Chun Loong3

2022-01-05

10.3791/63382

Jove-Journal of Visualized Experiments   影响因子和分区

1940-087X

1940-087X

Bacterial swarming motility is a common microbiological phenotype that bacterial communities use to migrate over semisolid surfaces. In investigations of induced swarming motility, specific concentration of an inducer may not be able to report events occurring within the optimal concentration range to elicit the desired responses from a species. Semisolid plates containing multiple concentrations are commonly used to investigate the response within an inducer concentration range. However, separate semisolid plates increase variations in medium viscosity and moisture content within each plate due to nonuniform solidification time. This paper describes a one-step method to simultaneously test surface swarming motility on a single gradient plate, where the isometrically arranged test wells allow the simultaneous acquisition of multiconcentration responses. In the present work, the surface swarming of Escherichia coli K12 and Pseudomonas aeruginosa PAO1 were evaluated in response to a concentration gradient of inducers such as resveratrol and arabinose. Periodically, the swarm morphologies were imaged using an imaging system to capture the entire surface swarming process. The quantitative measurement of the swarm morphologies was acquired using ImageJ software, providing analyzable information of the swarm area. This paper presents a simple gradient swarm plate method that provides qualitative and quantitative information about the inducers' effects on surface swarming, which can be extended to study the effects of other inducers on a broader range of motile bacterial species.

SCI

英语

第一

Ministry of Science and Technology's National Key RD plan[2018YF0902604] ; National Natural Science Foundation of China's Research Fund for International Young Scientists[22050410270] ; Shenzhen Institutes of Advanced Technology External Funds[DWKF20190001]

Science & Technology - Other Topics

Multidisciplinary Sciences

WOS:000798949800013

JOURNAL OF VISUALIZED EXPERIMENTS

2-s2.0-85123745516

Scopus

1.Department of Biomedical Engineering,Southern University of Science and Technology,
2.Department of Biomedical Engineering, Southern University of Science and Technology;
3.Department of Biomedical Engineering, Southern University of Science and Technology; hejl@sustech.edu.cn

Guo，Siyu,Liu，Zhao,Yang，Yongshuai,et al. Quantifying Bacterial Surface Swarming Motility on Inducer Gradient Plates[J]. Jove-Journal of Visualized Experiments,2022(179).

Guo，Siyu,Liu，Zhao,Yang，Yongshuai,Chen，Jun,&Ho，Chun Loong.(2022).Quantifying Bacterial Surface Swarming Motility on Inducer Gradient Plates.Jove-Journal of Visualized Experiments(179).

Guo，Siyu,et al."Quantifying Bacterial Surface Swarming Motility on Inducer Gradient Plates".Jove-Journal of Visualized Experiments .179(2022).