Human Urine-Derived Stem Cells Improve Partial Bladder Outlet Obstruction in Rats: Preliminary Data and microRNA-mRNA Expression Profile

Tu，Menjiang1; Wang，Rui1; Zhu，Pei1; Wang，Qingqing2; Sun，Bishao2; Lu，Keshi1; Zhang，Jiawei1; Xie，Weijie1; Guo，Huan1; Li，Shulin1; Wu，Yuqi1; Wang，Xiangwei1

2022

10.1007/s12015-022-10340-0

Stem Cell Reviews and Reports   影响因子和分区

2629-3269

2629-3277

Partial bladder outlet obstruction (pBOO) often results in bladder tissue inflammation and remodeling. As human urine-derived stem cells (USCs) have demonstrated therapeutic benefits, we used a rat model to investigate the effect of USCs on bladder function and explore the miRNA and gene expression profiles in bladder tissue using RNA sequencing. Eighteen rats were assigned to a sham surgery group, pBOO group, and pBOO+USC group (six biweekly treatments). Routine urodynamic monitoring, analysis of detrusor muscle strips, and pathophysiology assessments were conducted. Finally, altered miRNA and mRNA expression profiles of bladder tissue were examined using RNA sequencing and bioinformatics analysis. After USC treatment, elevated bladder compliance and maximal voiding pressure, declined end filling pressure and voided volume, and improved detrusor muscle contractility and carbachol sensitivity were found. Histology and TUNEL assay revealed reduced collagen deposition and muscle cell apoptosis in bladder tissue. The differential expression of eight miRNAs was reversed by USC treatment. Two large nodes (miR-142 and miR-9a) were identified in the miRNA-gene interaction network in the USC-treated group. The Kyoto Encyclopedia of Genes and Genomes analysis revealed enrichment of multiple significant pathways, including those involved in necroptosis and cytokine-cytokine receptor interactions. This is the first study to demonstrate the protective effect of USCs on bladder function and remodeling in pBOO rats. The miRNA and mRNA expression levels differed in the bladder of pBOO rats with and without USC treatment. Although the mechanism underlying these effects has not been fully elucidated, necroptosis and cytokine-cytokine receptor interaction-related pathways may be involved. Graphical abstract: [Figure not available: see fulltext.]

gene expression profiles human urine-derived stem cells microRNA miR-142 miR-9a partial bladder outlet obstruction

SCI

英语

第一 ; 通讯

WOS:000762888200003

2-s2.0-85125449223

Scopus

1.Department of Urology,Southern University of Science and Technology Hospital,Shenzhen,Liuxian Street, Nanshan District,NO.6019,China
2.Department of Urology,Second Affiliated Hospital,Third Military Medical University,Chongqing,China

Tu，Menjiang,Wang，Rui,Zhu，Pei,et al. Human Urine-Derived Stem Cells Improve Partial Bladder Outlet Obstruction in Rats: Preliminary Data and microRNA-mRNA Expression Profile[J]. Stem Cell Reviews and Reports,2022.

Tu，Menjiang.,Wang，Rui.,Zhu，Pei.,Wang，Qingqing.,Sun，Bishao.,...&Wang，Xiangwei.(2022).Human Urine-Derived Stem Cells Improve Partial Bladder Outlet Obstruction in Rats: Preliminary Data and microRNA-mRNA Expression Profile.Stem Cell Reviews and Reports.

Tu，Menjiang,et al."Human Urine-Derived Stem Cells Improve Partial Bladder Outlet Obstruction in Rats: Preliminary Data and microRNA-mRNA Expression Profile".Stem Cell Reviews and Reports (2022).