MicroRNA MiR-490-5p suppresses pancreatic cancer through regulating epithelial-mesenchymal transition via targeting MAGI2 antisense RNA 3

Xu，Zhenglei1; Chen，Zeming2; Peng，Minsi2; Zhang，Zhuliang2; Luo，Weixiang3; Shi，Ruiyue1; Wang，Lisheng1; Hong，Yingcai4

2022

10.1080/21655979.2021.2024653

Bioengineered   影响因子和分区

2165-5979

2165-5987

Pancreatic cancer with about 5% five-year overall survival rate remains a challenge. Invasion and migration of pancreatic cancer cells are the main factors leading to poor prognosis. MicroRNA-490-5p (miR-490-5p) has anti-cancer effects in a variety of tumors, but its role in pancreatic cancer has not been reported. The mRNA expressions of miR-490-5p, MAGI2 antisense RNA 3 (MAGI2-AS3), Matrix metalloproteinase (MMP)2, MMP9, N-cadherin, and E-cadherin were detected by quantitative real-time PCR, while the protein expressions of these genes except miR-490-5p were measured by Western blot analysis. The cell viability, apoptosis, migration and invasion were detected by cell counting kit-8 (CCK-8), apoptosis and transwell assays. MiR-490-5p was abnormally low-expressed in pancreatic cancer, whose down-regulation generated enhanced effects on viability, migration and invasion in pancreatic cancer cells, as well as MAGI2-AS3 expression. MiR-490-5p mimic exerted the opposite effect on cells, which also down-regulated MMP2, MMP9, and N-cadherin protein expressions, while up-regulating E-cadherin protein expression. MAGI2-AS3, which was the targeted binding site of miR-490-5p, promoted viability, migration and invasion, and inhibited apoptosis of cancer cells. More importantly, miR-490-5p played an anti-cancer role in pancreatic cancer by targeting MAGI2-AS3 and regulating epithelial–mesenchymal transition (EMT), which was partially offset by MAGI2-AS3.

Epithelial–mesenchymal transition MAGI2-AS3 MicroRNA-490-5p

SCI

英语

第一 ; 通讯

WOS:000744281800001

2-s2.0-85123369652

Scopus

1.Department of Gastroenterology,Shenzhen People’s Hospital (The Second Clinical Medical College,Jinan University,the First Affiliated Hospital,Southern University of Science and Technology,Guangdong,China
2.The Second Clinical Medical College,Jinan University,Guangdong,China
3.Department of Nursing,Shenzhen People’s Hospital (The Second Clinical Medical College,Jinan University,the First Affiliated Hospital,Southern University of Science and Technology,Guangdong,China
4.Department of Thoracic Surgery,Shenzhen People’s Hospital (The Second Clinical Medical College,Jinan University,the First Affiliated Hospital,Southern University of Science and Technology),Guangdong,China

Xu，Zhenglei,Chen，Zeming,Peng，Minsi,et al. MicroRNA MiR-490-5p suppresses pancreatic cancer through regulating epithelial-mesenchymal transition via targeting MAGI2 antisense RNA 3[J]. Bioengineered,2022,13(2):2673-2685.

Xu，Zhenglei.,Chen，Zeming.,Peng，Minsi.,Zhang，Zhuliang.,Luo，Weixiang.,...&Hong，Yingcai.(2022).MicroRNA MiR-490-5p suppresses pancreatic cancer through regulating epithelial-mesenchymal transition via targeting MAGI2 antisense RNA 3.Bioengineered,13(2),2673-2685.

Xu，Zhenglei,et al."MicroRNA MiR-490-5p suppresses pancreatic cancer through regulating epithelial-mesenchymal transition via targeting MAGI2 antisense RNA 3".Bioengineered 13.2(2022):2673-2685.