The Inhibitory Effect of miR-345 on Glioma Progression Is Closely Related to circRNA-hsa_circ_0073237 and HDGF

Xu，Wu1,2; Che，Dan Dan3; Liu，Qing1; Pan，Ya Wen4; Lv，Sheng Qing5; Chen，Bao Dong6

2021-12-01

10.1159/000518667

CELLS TISSUES ORGANS   影响因子和分区

1422-6405

1422-6421

Glioma is the most common primary malignant tumor of the central nervous system and has a poor prognosis. Therefore, exploring the key molecular targets is a new opportunity for basic research and clinical treatment of glioma. Previous studies found that circRNA-hsa_circ_0073237 was upregulated in gliomas. Our further analyses of the biological function and molecular mechanism of hsa_circ_0073237 showed that hsa_circ_0073237 was also upregulated in glioma cell lines and could combine with miR-345 to inhibit its expression. miR-345 was downregulated in glioma tissues and cells, and targeted to regulate the expression of hepatoma-derived growth factor (HDGF), while HDGF expression was enhanced in glioma. Hsa_circ_0073237 promoted the expression of HDGF in glioma cells by adsorbing miR-345. Hsa_circ_0073237 siRNA, miR-345, and HDGF siRNA effectively inhibited cell viability and invasion and promoted cell apoptosis. When expression of hsa_circ_0073237 and miR-345 was inhibited simultaneously, cell viability, apoptosis, and invasion did not change significantly; however, after transfection with HDGF overexpression vector, the effects of hsa_circ_0073237 siRNA and miR-345 on glioma cell viability, apoptosis, and invasion were obviously reversed. Further construction of glioma xenograft models in nude mice confirmed that the introduction of miR-345 in vivo effectively inhibited tumor growth, significantly reduced tumor diameter and weight, and obviously decreased the expression of HDGF. Therefore, hsa_circ_0073237 can regulate the biological functions of glioma cells through miR-345/HDGF, thereby affecting the progression of tumors, indicating that the hsa_circ_0073237/miR-345/HDGF pathway may be a key target for the treatment of glioma.

Central nervous system circRNA Glioma Hepatoma-derived growth factor miR-345

SCI

英语

其他

WOS:000765982500005

MOLECULAR BIOLOGY & GENETICS

2-s2.0-85121982153

Scopus

1.Department of Neurosurgery,Xiangya Hospital,Central South University,Changsha,China
2.Department of Neurosurgery,The Affiliated Hospital,Jiujiang University,Jiujiang,China
3.Department of Intensive Care Unit,The Third People's Hospital of Shenzhen,The Second Affiliated Hospital,Southern University of Science and Technology,Shenzhen,China
4.Department of Neurosurgery,The Second Hospital,Lanzhou University,Lanzhou,China
5.Department of Neurosurgery,Xinqiao Hospital,Third Military Medical University,Chongqing,China
6.Department of Neurosurgery,Shenzhen Hospital,Peking University,Shenzhen,China

Xu，Wu,Che，Dan Dan,Liu，Qing,et al. The Inhibitory Effect of miR-345 on Glioma Progression Is Closely Related to circRNA-hsa_circ_0073237 and HDGF[J]. CELLS TISSUES ORGANS,2021,210(5-6):368-379.

Xu，Wu,Che，Dan Dan,Liu，Qing,Pan，Ya Wen,Lv，Sheng Qing,&Chen，Bao Dong.(2021).The Inhibitory Effect of miR-345 on Glioma Progression Is Closely Related to circRNA-hsa_circ_0073237 and HDGF.CELLS TISSUES ORGANS,210(5-6),368-379.

Xu，Wu,et al."The Inhibitory Effect of miR-345 on Glioma Progression Is Closely Related to circRNA-hsa_circ_0073237 and HDGF".CELLS TISSUES ORGANS 210.5-6(2021):368-379.