南方科技大学知识苑(SUSTech KC): Helicobacter pylori infection affects the human gastric microbiome, as revealed by metagenomic sequencing

题名	Helicobacter pylori infection affects the human gastric microbiome, as revealed by metagenomic sequencing
作者	Wang, Daoming 1; Zhang, Tongda 1; Lu, Yueqi 1; Wang, Changzheng 1; Wu, Yumei 2; Li, Jiandong 1; Tao, Ye 1; Deng, Le 2; Zhang, Xiaoyin 2; Ma, Jinmin 1
通讯作者	Zhang, Xiaoyin; Ma, Jinmin
发表日期	2022-03-01
DOI	10.1002/2211-5463.13390
发表期刊	FEBS Open Bio 影响因子和分区
ISSN	2211-5463
摘要	Helicobacter pylori infection is a prevalent infectious disease, associated with many gastric diseases, including gastritis, gastric ulcer, and gastric cancer. To reveal the characteristics of the gastric microbiome in patients infected with H. pylori, we performed metagenomic shotgun sequencing of stomach swab samples from 96 patients and then conducted metagenomic association analyses between alterations in the gastric microbiome and H. pylori infection status. The overall composition of the gastric microbiota in H. pylori-infected individuals was distinctly different from the negative controls; H. pylori became the dominant species after colonizing the human stomach and significantly decreased the alpha-diversity of the gastric community (P < 0.05, Wilcoxon rank-sum test). We also identified 6 HPI-associated microbial species (FDR < 0.05, Wilcoxon rank-sum test): Stenotrophomonas maltophilia, Stenotrophomonas unclassified, Chryseobacterium unclassified, Pedobacter unclassified, Variovorax unclassified, and Pseudomonas stutzeri. Furthermore, 55 gastric microbial pathways were enriched in the H. pylori-positive group, whereas only 2 pathways were more abundant in the H. pylori-negative group: dTDP-L-rhamnose biosynthesis and tetrapyrrole biosynthesis (FDR < 0.05, Wilcoxon rank-sum test). Gastritis was not associated with non-H. pylori species in the stomach (P > 0.05, Wilcoxon rank-sum test). This study revealed alterations in gastric microbial taxa and function associated with HPI in the Chinese population, which provides an insight into gastric microbial interactions and their potential role in the pathological process of gastric diseases.
关键词	gastric diseases gastric microbiome gastric ulcer gastritis Helicobacter pylori infection metagenome
相关链接	[来源记录]
收录类别	SCI
语种	英语
学校署名	通讯
资助项目	Science, Technology and Innovation Commission of Shenzhen Municipality[JCYJ20170412153155228]
WOS研究方向	Biochemistry & Molecular Biology
WOS类目	Biochemistry & Molecular Biology
WOS记录号	WOS:000772728500001
出版者	WILEY
来源库	Web of Science
引用统计	被引频次[WOS]：13
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/328457
专题	南方科技大学第二附属医院
作者单位	1.BGI Shenzhen, Shenzhen 518083, Peoples R China 2.Southern Univ Sci & Technol, Natl Clin Res Ctr Infect Dis, Peoples Hosp Shenzhen 3, Dept Gastroenterol,Affiliated Hosp 2, Shenzhen 518114, Peoples R China
通讯作者单位	南方科技大学第二附属医院
推荐引用方式 GB/T 7714	Wang, Daoming,Zhang, Tongda,Lu, Yueqi,et al. Helicobacter pylori infection affects the human gastric microbiome, as revealed by metagenomic sequencing[J]. FEBS Open Bio,2022.
APA	Wang, Daoming.,Zhang, Tongda.,Lu, Yueqi.,Wang, Changzheng.,Wu, Yumei.,...&Ma, Jinmin.(2022).Helicobacter pylori infection affects the human gastric microbiome, as revealed by metagenomic sequencing.FEBS Open Bio.
MLA	Wang, Daoming,et al."Helicobacter pylori infection affects the human gastric microbiome, as revealed by metagenomic sequencing".FEBS Open Bio (2022).