Propofol-induced sleep ameliorates cognition impairment in sleep-deprived rats

Lu, Yizhi1; Xiao, Yong1; Tu, Youbing2; Dai, Weixin1; Xie, Yubo1

2022-03-01

10.1007/s11325-022-02591-5

Sleep and Breathing   影响因子和分区

1520-9512

1522-1709

Purpose Propofol has been shown to clear sleep debt in rats after sleep deprivation (SD). We examined whether or not propofol-assisted sleep can restore cognitive function in SD rats and explored the possible mechanisms. Methods A sleep deprivation model was established by housing 9 to 12 week-old rats to a multiplatform water tank for 96 h. Model rats were then intraperitoneally injected with different concentrations of propofol or 10% fat emulsion (vehicle control). All treatment groups were examined for spatial learning and memory ability in the Morris water maze (MWM). After euthanasia, morphological changes in the hippocampus, hippocampal neurons, and mitochondria were examined by hematoxylin-eosin staining and transmission electron microscopy. Serum and hippocampal levels of IL-1 beta, TNF-alpha, and hippocampal concentrations of ATP and Cyt-c were measured by ELISA (enzyme-linked immunosorbent assay). Immunohistochemistry and Western blotting were performed to assess hippocampal expression of Bcl-2, Bax, and cleaved caspase-3. Results Results showed that escape latencies in MWM training trials were significantly shorter and target crossings in the memory probe trial significantly greater in propofol-treated SD model rats compared to vehicle-treated SD rats. Propofol also reduced the number of apoptotic bodies in the hippocampal CA1 region. Sleep deprivation reduced IL-1 beta and ATP in hippocampus while increasing TNF-alpha and Cyt-c, and propofol treatment reversed all these changes. There was no significant difference in Bcl-2 expression between propofol- and vehicle-treated SD rats, but pro-apoptotic Bax and cleaved caspase-3 expression levels were significantly reduced by propofol in SD rats. Conclusions Propofol-assisted sleep restored cognitive function in SD rats possibly by attenuating mitochondria-mediated neuronal apoptosis in the hippocampus.

Propofol Sleep deprivation Cognitive function Apoptosis

SCI

英语

其他

Program of Guangxi Key Research and Development[AB18221031] ; Key Project of Guangxi Natural Science Foundation[2020GXNSFDA238025] ; National Natural Science Foundation of China[81373498]

Neurosciences & Neurology ; Respiratory System

Clinical Neurology ; Respiratory System

WOS:000771536200001

SPRINGER HEIDELBERG

Web of Science

1.Guangxi Med Univ, Affiliated Hosp 1, Dept Anesthesiol, 6 Shuang Yong Rd, Nanning 530021, Guangxi, Peoples R China
2.Southern Univ Sci & Technol, Affiliated Hosp 1, Shenzhen Peoples Hosp, Dept Anesthesiol, Shenzhen 518020, Guangdong, Peoples R China

Lu, Yizhi,Xiao, Yong,Tu, Youbing,et al. Propofol-induced sleep ameliorates cognition impairment in sleep-deprived rats[J]. Sleep and Breathing,2022,27(1).

Lu, Yizhi,Xiao, Yong,Tu, Youbing,Dai, Weixin,&Xie, Yubo.(2022).Propofol-induced sleep ameliorates cognition impairment in sleep-deprived rats.Sleep and Breathing,27(1).

Lu, Yizhi,et al."Propofol-induced sleep ameliorates cognition impairment in sleep-deprived rats".Sleep and Breathing 27.1(2022).