Acceptor engineering of small-molecule fluorophores for NIR-II fluorescence and photoacoustic imaging

Li，Yaxi1; Zhou，Hongli2; Bi，Renzhe3; Li，Xiuting3; Zha，Menglei1; Yang，Yanqing2; Ni，Jen Shyang1; Liew，Weng Heng4; Olivo，Malini3; Yao，Kui4; Liu，Jie2; Chen，Hao5; Li，Kai1

2021-12-28

10.1039/d1tb02282b

Journal of Materials Chemistry B   影响因子和分区

2050-750X

2050-7518

Fluorescence imaging in the second near-infrared window (NIR-II) has been an emerging technique in diversein vivoapplications with high sensitivity/resolution and deep tissue penetration. To date, the design principle of the reported NIR-II organic fluorophores has heavily relied on benzo[1,2-c:4,5-c′]bis([1,2,5]thiadiazole) (BBTD) as a strong electron acceptor. Here, we report the rational design and synthesis of a NIR-II fluorescent molecule with the rarely used [1,2,5]thiadiazolo[3,4-f]benzotriazole (TBZ) core to replace BBTD as the electron acceptor. Thanks to the weaker electron deficiency of the TBZ core than BBTD, the newly yielded NIR-II molecule (BTB) based nanoparticles have a higher mass extinction coefficient and quantum yield in water. In contrast, the nanoparticle suspension of its counterpart with BBTD as the core is nearly nonemissive. The NIR-II BTB nanoparticles allow video-rate fluorescence imaging for vasculature imaging in ears, hindlimbs, and the brain of the mouse. Additionally, its large absorptivity in the NIR-I region also promotes bioimaging using photoacoustic microscopy (PAM) and tomography (PAT). Upon surface conjugation with the Arg-Gly-Asp (RGD) peptide, the functionalized nanoparticles ensured targeted detection of integrin-overexpressed tumors through both imaging modalities in two- and three-dimensional views. Thus, our approach to engineering acceptors of organic fluorophores offers a promising molecular design strategy to afford new NIR-II fluorophores for versatile biomedical imaging applications.

SCI ; EI

英语

第一

WOS:000725115100001

20215211386796

Fluorescence imaging ; Fluorophores ; Medical imaging ; Molecules ; Nanoparticles ; Photoacoustic effect ; Synthesis (chemical)

Biomedical Engineering:461.1 ; Light/Optics:741.1 ; Imaging Techniques:746 ; Acoustic Waves:751.1 ; Nanotechnology:761 ; Physical Chemistry:801.4 ; Chemical Reactions:802.2 ; Atomic and Molecular Physics:931.3 ; Solid State Physics:933

2-s2.0-85121585190

Scopus

1.Shenzhen Key Laboratory of Smart Healthcare Engineering,Department of Biomedical Engineering,Southern University of Science and Technology,Shenzhen,518055,China
2.Key Laboratory of Flexible Electronics (KLOFE) Institute of Advanced Materials (IAM),Nanjing Tech University (Nanjing Tech),Nanjing,30 South Puzhu Road,211800,China
3.Institute of Bioengineering and Bioimaging (IBB),A*STAR (Agency for Science,Technology and Research),11 Biopolis Way,Singapore
4.Institute of Materials Research and Engineering,A*STAR (Agency for Science,Technology and Research),2 Fusionopolis Way, Innovis,138634,Singapore
5.Molecular Imaging Center,Shanghai Institute of Materia Medica,Chinese Academy of Sciences,Shanghai,201203,China

Li，Yaxi,Zhou，Hongli,Bi，Renzhe,et al. Acceptor engineering of small-molecule fluorophores for NIR-II fluorescence and photoacoustic imaging[J]. Journal of Materials Chemistry B,2021,9(48):9951-9960.

Li，Yaxi.,Zhou，Hongli.,Bi，Renzhe.,Li，Xiuting.,Zha，Menglei.,...&Li，Kai.(2021).Acceptor engineering of small-molecule fluorophores for NIR-II fluorescence and photoacoustic imaging.Journal of Materials Chemistry B,9(48),9951-9960.

Li，Yaxi,et al."Acceptor engineering of small-molecule fluorophores for NIR-II fluorescence and photoacoustic imaging".Journal of Materials Chemistry B 9.48(2021):9951-9960.