Automated Centrifugal Microfluidic Chip Integrating Pretreatment and Molecular Diagnosis for Hepatitis B Virus Genotyping from Whole Blood

Zong，Nan; Gao，Ying; Chen，Yong; Luo，Xueqing; Jiang，Xingyu

2022-03-29

10.1021/acs.analchem.2c00337

ANALYTICAL CHEMISTRY   影响因子和分区

0003-2700

1520-6882

Point-of-care (POC) testing for nucleic acid that combines pretreatment and molecular diagnosis is crucial in analyzing complex samples such as those encountered in clinical diagnosis. Herein, we developed a centrifugal microfluidic platform, which can achieve a series of functions including separating serum and adsorbing, washing, eluting, and detecting DNA. We combined multiple signal enhancement systems including recombinase polymerase amplification (RPA), T7 transcription technology, and clustered regularly interspaced short palindromic repeat (CRISPR) technology to yield an ultrabright signal, which can avoid false-negative results. As an application, hepatitis B virus (HBV), a virus that causes global public health problems, was successfully detected and genotyped from whole blood on the automated centrifugal microfluidic platform. Compared to the traditional diagnosis process, the POC platform largely decreased the consumption of time from 3 to 1 h and the consumption of professional labor from three persons to only one. The automated centrifugal microfluidic platform integrated pretreatment and molecular diagnosis will play an essential role in clinical detection.

SCI ; EI

英语

NI论文

第一 ; 通讯

National Key R&D Program of China[2018YFA0902600] ; National Natural Science Foundation of China[21535001,81730051,21761142006] ; Guangdong Province[2019ZT08Y191] ; City of Shenzhen[KQTD20190929172743294]

Chemistry

Chemistry, Analytical

WOS:000779305600040

AMER CHEMICAL SOC

20221411883702

Automation ; Blood ; Centrifugation ; Microfluidics ; Molecular biology ; Viruses

Biological Materials and Tissue Engineering:461.2 ; Medicine and Pharmacology:461.6 ; Biology:461.9 ; Microfluidics:632.5.1 ; Automatic Control Principles and Applications:731 ; Chemical Operations:802.3

CHEMISTRY

2-s2.0-85127401260

Scopus

Shenzhen Key Laboratory of Smart Healthcare Engineering,Department of Biomedical Engineering,Southern University of Science and Technology,Shenzhen,No. 1088, Xueyuan Rd., Xili, Nanshan District, Guangdong,518055,China

Zong，Nan,Gao，Ying,Chen，Yong,et al. Automated Centrifugal Microfluidic Chip Integrating Pretreatment and Molecular Diagnosis for Hepatitis B Virus Genotyping from Whole Blood[J]. ANALYTICAL CHEMISTRY,2022,94(12):5196-5203.

Zong，Nan,Gao，Ying,Chen，Yong,Luo，Xueqing,&Jiang，Xingyu.(2022).Automated Centrifugal Microfluidic Chip Integrating Pretreatment and Molecular Diagnosis for Hepatitis B Virus Genotyping from Whole Blood.ANALYTICAL CHEMISTRY,94(12),5196-5203.

Zong，Nan,et al."Automated Centrifugal Microfluidic Chip Integrating Pretreatment and Molecular Diagnosis for Hepatitis B Virus Genotyping from Whole Blood".ANALYTICAL CHEMISTRY 94.12(2022):5196-5203.