C. acnes qPCR-Based Antibiotics Resistance Assay (ACQUIRE) Reveals Widespread Macrolide Resistance in Acne Patients and Can Eliminate Macrolide Misuse in Acne Treatment

Zhang，Jingheng1; Yu，Fang1; Fu，Keyun1; Ma，Xinyu1; Han，Yi1; Ali，Chi Ching1; Zhou，Haonan2; Xu，Yantao1,3; Zhang，Tingyue1; Kang，Shuntong1; Xu，Yiming1; Li，Zhuolin2; Shi，Jiaqi2; Gao，Shuai2; Chen，Yongyi4,5; Chen，Liyu6; Zhang，Jianglin7,8,9; Zhu，Feizhou2,5,10

2022

10.3389/fpubh.2022.787299

Frontiers in public health   影响因子和分区

2296-2565

Background: Macrolides have been widely used to treat moderate-to-severe acne for more than 50 years. However, the prevalent antibiotic resistance of Propionibacterium acnes, along with the absence of clinically available resistance tests, has made macrolide misuse a frequent occurrence around the globe, with serious consequences. Objective: We developed Cutibacterium acnes quantitative PCR (qPCR)-based antibiotics resistance assay (ACQUIRE) to enable fast and accurate detection of C. acnes macrolide resistance in clinical settings, representing an opportunity to administer antibiotics more wisely and improve the quality of care. Methods: A cross-sectional observational study (n = 915) was conducted to probe into the macrolide resistance of C. acnes in patients with acne. Results: The high sensitivity of ACQUIRE enabled us to reveal a much higher C. acnes 23S recombinant DNA (rDNA) point mutation rate (52%) and thus a higher macrolide resistance (75.5%) compared to previous reports. Carriage of ermX gene was discovered on 472 (53%) subjects, which concurs with previous studies. Conclusion: The macrolide resistance of C. acnes is much higher than previously reported. Integrating ACQUIRE into acne treatment modalities may eliminate macrolide misuse and achieve better clinical improvements.

acne (acne vulgaris) antimicrobial antimicrobial resistance macrolide-resistant gene quantitative PCR (qPCR)

SCI ; SSCI

英语

其他

China Hunan Provincial Science and Technology Plan["2018SK7005","2017SK2092"] ; Innovative Education Reform Program of Central South University[2019CG045] ; National Undergraduate Innovation Training Program of Central South University[201810533233,"GS201910533150X"]

Public, Environmental & Occupational Health

Public, Environmental & Occupational Health

WOS:000791547700001

FRONTIERS MEDIA SA

2-s2.0-85127479342

Scopus

1.Xiangya School of Medicine,Central South University,Changsha,China
2.Department of Biochemistry and Molecular Biology,School of Life Sciences,Central South University,Changsha,China
3.Department of Dermatology,Xiangya Hospital,Central South University,Changsha,China
4.Hunan Cancer Hospital,Affiliated Cancer Hospital of Xiangya School of Medicine,Central South University,Changsha,China
5.Clinical Research Center for Wound Healing in Hunan Province,Changsha,China
6.Department of Microbiology,School of Basic Medical Sciences,Central South University,Changsha,China
7.Department of Dermatology,Shenzhen People's Hospital,Shenzhen,China
8.Second Clinical Medical College,Jinan University,Guangzhou,China
9.First Affiliated Hospital,Southern University of Science and Technology,Shenzhen,China
10.Hunan Key Laboratory of Animal Models for Human Diseases,Changsha,China

Zhang，Jingheng,Yu，Fang,Fu，Keyun,et al. C. acnes qPCR-Based Antibiotics Resistance Assay (ACQUIRE) Reveals Widespread Macrolide Resistance in Acne Patients and Can Eliminate Macrolide Misuse in Acne Treatment[J]. Frontiers in public health,2022,10.

Zhang，Jingheng.,Yu，Fang.,Fu，Keyun.,Ma，Xinyu.,Han，Yi.,...&Zhu，Feizhou.(2022).C. acnes qPCR-Based Antibiotics Resistance Assay (ACQUIRE) Reveals Widespread Macrolide Resistance in Acne Patients and Can Eliminate Macrolide Misuse in Acne Treatment.Frontiers in public health,10.

Zhang，Jingheng,et al."C. acnes qPCR-Based Antibiotics Resistance Assay (ACQUIRE) Reveals Widespread Macrolide Resistance in Acne Patients and Can Eliminate Macrolide Misuse in Acne Treatment".Frontiers in public health 10(2022).