The virota and its transkingdom interactions in the healthy infant gut

Beller, Leen1; Deboutte, Ward1; Vieira-Silva, Sara2,3; Falony, Gwen2,3; Tito, Raul Yhossef2,3; Rymenans, Leen2,3; Yinda, Claude Kwe4; Vanmechelen, Bert5; Van Espen, Lore1; Jansen, Daan1; Shi, Chenyan1,6,7,8; Zeller, Mark9; Maes, Piet5; Faust, Karoline2; Van Ranst, Marc5; Raes, Jeroen2,3; Matthijnssens, Jelle1

2022-03-29

10.1073/pnas.2114619119

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   影响因子和分区

0027-8424

1091-6490

Virome and 16/18S analyses were performed on 304 longitudinal fecal samples of eight infants. The gut virota-the collection of all viruses present in the gut-was dominated by bacteriophages, which were nearly absent at birth and emerged rapidly within the first weeks after birth. Over 85% of phage reads correspond to 305 near-complete genomes, most of which (70.5%) were individual infant-specific, including two crAs-sphages, whereas 7.8% of phages were present in at least 50% of infants. Bacterial hosts could be predicted for 80% of phages, mainly infecting Firmicutes. Strong temporal correlations between phages and their predicted bacterial hosts were identified for >40% of our phages, and together with the observation of a decreasing fraction of phages with a temperate lifestyle further suggest that phages are induced from early-colonizing bacteria. The vast majority (>86%) of identified eukaryotic viruses, known to cause gastroenteritis, occurred without clinical signs, and an increase in the rate of infection occurred after day-care entrance. On average, 112 genomic contigs of distinct anelloviruses could be identified per infant, some of which were shed at >1 y. The identified plant viruses reflected the infant diet. Finally, the sporadic identification of fungi and parasites argues against the presence of such stable communities in the study population. Overall, this work provides a very high temporal resolution on how the different members of the infant gut microbiota, and especially the virome, develop over time in the gut of healthy infants, and might serve as valuable baseline knowledge for further studies investigating the effect of perturbations in the infant gut microbiota.

infant virota microbiota virome transkingdom

SCI

英语

NI论文

其他

Fonds Wetenschappelijk Onderzoek (FWO; Research Foundation Flanders)["1S61618N","1234321N","1S28617N","1S25720N","1S78021N"] ; KU Leuven Grant[OT-14-113]

Science & Technology - Other Topics

Multidisciplinary Sciences

WOS:000780677900004

NATL ACAD SCIENCES

BIOLOGY & BIOCHEMISTRY;CLINICAL MEDICINE;MULTIDISCIPLINARY;PLANT & ANIMAL SCIENCE;ENVIRONMENT/ECOLOGY;SOCIAL SCIENCES, GENERAL;MICROBIOLOGY;ECONOMICS BUSINESS;IMMUNOLOGY;MATERIALS SCIENCE;MATHEMATICS;SPACE SCIENCE;MOLECULAR BIOLOGY & GENETICS;PHARMACOLOGY & TOXICOLOGY;CHEMISTRY;PSYCHIATRY/PSYCHOLOGY;NEUROSCIENCE & BEHAVIOR;PHYSICS;GEOSCIENCES;AGRICULTURAL SCIENCES;ENGINEERING

Web of Science

1.Univ Leuven, Rega Inst, Dept Microbiol Immunol & Transplantat, Lab Viral Metagen, B-3000 Leuven, Belgium
2.Univ Leuven, Rega Inst, Dept Microbiol Immunol & Transplantat, Lab Mol Bacteriol, B-3000 Leuven, Belgium
3.Flemish Inst Biotechnol VIB, Ctr Microbiol, B-3000 Leuven, Belgium
4.Natl Inst Allergy & Infect Dis NIAID, Virus Ecol Unit, Lab Virol, Rocky Mt Labs,NIH, Hamilton, MT 59840 USA
5.Univ Leuven, Rega Inst, Dept Microbiol Immunol & Transplantat, Lab Clin & Epidemiol Virol, B-3000 Leuven, Belgium
6.Jinan Univ, Ctr Lab Longhua Branch, Shenzhen Peoples Hosp, Clin Med Coll 2, Guangzhou, Peoples R China
7.Southern Univ Sci & Technol, Affiliated Hosp 1, Shenzhen 518020, Guangdong, Peoples R China
8.Jinan Univ, Clin Med Coll 2, Shenzhen Peoples Hosp, Dept Infect Dis, Guangzhou, Peoples R China
9.Scripps Res, Dept Immunol & Microbiol, La Jolla, CA 92037 USA

Beller, Leen,Deboutte, Ward,Vieira-Silva, Sara,et al. The virota and its transkingdom interactions in the healthy infant gut[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2022,119(13).

Beller, Leen.,Deboutte, Ward.,Vieira-Silva, Sara.,Falony, Gwen.,Tito, Raul Yhossef.,...&Matthijnssens, Jelle.(2022).The virota and its transkingdom interactions in the healthy infant gut.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,119(13).

Beller, Leen,et al."The virota and its transkingdom interactions in the healthy infant gut".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 119.13(2022).