A facile way to construct Sr-doped apatite coating on the surface of 3D printed scaffolds to improve osteogenic effect

Chen, Shangsi1; Wang, Yue2; Ma, Jun1

2022-04-01

10.1177/08853282221087107

JOURNAL OF BIOMATERIALS APPLICATIONS   影响因子和分区

0885-3282

1530-8022

Bone-like apatite coating fabricated by biomineralization process is a facile way for surface modification of porous scaffolds to improve interfacial behaviors and thus facilitate cell attachment, proliferation, and differentiation for bone tissue engineering. In this study, a Sr-containing calcium phosphate solution was made and used to construct a thick layer of Sr-doped bone-like apatite on the surface of 3D printed scaffolds via biomineralization process. Importantly, Sr-doped bone-like apatite could form and fully cover the 3D printed scaffolds surface in hours. The characterization results indicated that Sr2+ ions successfully replaced Ca2+ ions in bone-like apatite and the molar ratio of Sr/(Ca+Sr) was up to 8.2%. Furthermore, the Sr-doped apatite coating increased the compressive strength and Young's modulus of composite scaffolds. The compatibility and bioactivity of mineralized scaffolds were evaluated by cell attachment, proliferation, and differentiation of MC3T3-E1 cells. It was found that Sr-doped apatite coating could gradually release Sr2+ ions and further promote cell attachment, proliferation rate, and the expression of alkaline phosphatase activity and osteogenic related genes, such as collagen type I (Col I), Runt-related transcription factor 2 (Runx-2), osteopontin, and osterix. Therefore, the Sr-doped apatite coating fabricated by this facile and rapid biomineralization process offers a new strategy to modify 3D printed porous scaffolds with significantly improved mechanical and biological properties for bone tissue engineering applications.

3D printing biomineralization surface modification mechanical properties osteogenic effect

SCI ; EI

英语

其他

National Natural Science Foundation of China[31570965]

Engineering ; Materials Science

Engineering, Biomedical ; Materials Science, Biomaterials

WOS:000780705400001

SAGE PUBLICATIONS LTD

20221812062886

Apatite ; Biomechanics ; Biomineralization ; Bone ; Calcium phosphate ; Cell engineering ; Coatings ; Compressive strength ; Cytology ; Ions ; Phosphatases ; Scaffolds (biology) ; Strontium compounds ; Transcription

Biomedical Engineering:461.1 ; Biological Materials and Tissue Engineering:461.2 ; Biomechanics, Bionics and Biomimetics:461.3 ; Biology:461.9 ; Minerals:482.2 ; Printing Equipment:745.1.1 ; Biochemistry:801.2 ; Chemical Products Generally:804 ; Inorganic Compounds:804.2 ; Coating Materials:813.2

CLINICAL MEDICINE

Web of Science

1.Huazhong Univ Sci & Technol, Biomed Engn, East 11,Luoyu 1037, Wuhan 430074, Peoples R China
2.Southern Univ Sci & Technol, Dept Mech & Energy Engn, Shenzhen, Peoples R China

Chen, Shangsi,Wang, Yue,Ma, Jun. A facile way to construct Sr-doped apatite coating on the surface of 3D printed scaffolds to improve osteogenic effect[J]. JOURNAL OF BIOMATERIALS APPLICATIONS,2022,37:344-354.

Chen, Shangsi,Wang, Yue,&Ma, Jun.(2022).A facile way to construct Sr-doped apatite coating on the surface of 3D printed scaffolds to improve osteogenic effect.JOURNAL OF BIOMATERIALS APPLICATIONS,37,344-354.

Chen, Shangsi,et al."A facile way to construct Sr-doped apatite coating on the surface of 3D printed scaffolds to improve osteogenic effect".JOURNAL OF BIOMATERIALS APPLICATIONS 37(2022):344-354.