FAM107A as a Tumor Suppressor in Bladder Cancer Inhibits Cell Proliferation, Migration, and Invasion

Ou，Longhua1; Wei，Zhengmao2; Xu，Jinming1; Li，Weiqing3; Zhou，Yingchen4; Wang，Yan5; Shi，Bentao6,7

2022-03-01

ANNALS OF CLINICAL AND LABORATORY SCIENCE   影响因子和分区

0091-7370

1550-8080

OBJECTIVE: Bladder cancer (BC) is the most common cancer in urinary system. Recently, the function of family with sequence similarity 107 member A (FAM107A) has been reported in several carcinomas. This study aimed to reveal the potential role of FAM107A in bladder cancer. METHODS: Bioinformatics analysis was performed to assess the expression level of FAM107A in BC tissues and adjacent tumor-free bladder tissues. The results were confirmed by quantitative real-time polymerase chain reaction (RT-qPCR), western blot and immunohistochemistry staining in BC tissues and adjacent tumor-free bladder tissues as well as BC cell lines. In addition, plasmid was constructed to increase FAM107A protein level in BC cell lines. The effect of FM107A on cell growth, cell migration and invasion were analyzed by CCK8 assay, wound healing assay and transwell-invasion assay. RESULTS: The data showed that FAM107A was remarkably down-regulated in bladder cancer tissues and bladder cancer cell lines. Besides, low FAM107A expression was associated with high tumor grade of patients with bladder cancer. Moreover, the restoration of FAM107A remarkably suppressed the cell growth, migration, and invasion of BC cells. CONCLUSION: In summary, FAM107A might serve as a tumor suppressor which inhibits BC cell proliferation, migration, and invasion. This study suggests that FAM107A can be a candidate new diagnostic marker and possible therapeutic target gene of bladder cancer.

bladder cancer cell invasion cell migration cell proliferation FAM107A

SCI

英语

其他

Science, Technology and Innovation Commission of Shenzhen Municipality["JCYJ20180228175430794","JCYJ20190809104411245"] ; Sanming Project of Medicine in Shenzhen[SZSM202111007]

Medical Laboratory Technology

Medical Laboratory Technology

WOS:000853675000012

ASSOC CLINICAL SCIENTISTS

CLINICAL MEDICINE

2-s2.0-85128138367

Scopus

1.Department of Urology,Shenzhen Second People's Hospital,First Affiliated Hospital of Shenzhen University Health Science Center,Shenzhen,China
2.Department of Urology,Southern University of Science and Technology Hospital,Shenzhen,China
3.Department of General Surgery,Karamay Central Hospital,Karamay,China
4.Department of Surgery,Zhongshan Hospital of Traditional Chinese Medicine,Zhongshan,China
5.Department of Urology,Peking University Shenzhen Hospital,Shenzhen PKU-HKUST Medical Center,Shenzhen,China
6.Department of Urology,Shenzhen Second People's Hospital,First Affiliated Hospital of Shenzhen University Health Science Center,Shenzhen,China
7.Department of Urology,Peking University Shenzhen Hospital,Shenzhen PKU-HKUST Medical Center,Shenzhen,China

Ou，Longhua,Wei，Zhengmao,Xu，Jinming,et al. FAM107A as a Tumor Suppressor in Bladder Cancer Inhibits Cell Proliferation, Migration, and Invasion[J]. ANNALS OF CLINICAL AND LABORATORY SCIENCE,2022,52(2):260-268.

Ou，Longhua.,Wei，Zhengmao.,Xu，Jinming.,Li，Weiqing.,Zhou，Yingchen.,...&Shi，Bentao.(2022).FAM107A as a Tumor Suppressor in Bladder Cancer Inhibits Cell Proliferation, Migration, and Invasion.ANNALS OF CLINICAL AND LABORATORY SCIENCE,52(2),260-268.

Ou，Longhua,et al."FAM107A as a Tumor Suppressor in Bladder Cancer Inhibits Cell Proliferation, Migration, and Invasion".ANNALS OF CLINICAL AND LABORATORY SCIENCE 52.2(2022):260-268.