Global Proteomic Analyses Reveals Abnormal Immune Regulation in Patients With New Onset Ankylosing Spondylitis

Yu, Zongchao1; Hong, Xiaoping2; Zhang, Xiaoli3; Zheng, Fengping2; Liu, Fanna1; Xu, Huixuan2; Zhu, Chengxin2; Cai, Wanxia2; Liu, Dongzhou1; Yin, Lianghong1; Hu, Bo1; Tang, Donge2; Dai, Yong2

2022-03-17

10.3389/fimmu.2022.838891

Frontiers in Immunology   影响因子和分区

1664-3224

BackgroundAnkylosing spondylitis (AS) is a chronic inflammatory disease with serious consequences and a high rate of morbidity and mortality, In our previous work, we reveal the key features of proteins in new-onset ankylosing spondylitis patients. Material and MethodsAnkylosing spondylitis (AS) is a chronic inflammatory condition that affects the spine, and inflammation plays an essential role in AS pathogenesis. The inflammatory process in AS, however, is still poorly understood due to its intricacy. Systematic proteomic and phosphorylation analyses of peripheral blood mononuclear cells (PBMCs) were used to investigate potential pathways involved in AS pathogenesis. ResultsLiquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis was performed and discovered 782 differentially expressed proteins (DEPs) and 122 differentially phosphorylated proteins (DPPs) between 9 new-onset AS patients and 9 healthy controls. The DEPs were further verified using parallel reaction monitoring (PRM) analysis. PRM analysis verified that 3 proteins (HSP90AB1, HSP90AA1 and HSPA8) in the antigen processing and presentation pathway, 6 proteins (including ITPR1, MYLK and STIM1) in the platelet activation pathway and 10 proteins (including MYL12A, MYL9 and ROCK2) in the leukocyte transendothelial migration pathway were highly expressed in the PBMCs of AS patients. ConclusionThe key proteins involved in antigen processing and presentation, platelet activation and leukocyte transendothelial migration revealed abnormal immune regulation in patients with new-onset AS. These proteins might be used as candidate markers for AS diagnosis and new therapeutic targets, as well as elucidating the pathophysiology of AS.

ankylosing spondylitis (AS) global proteomic immune regulation peripheral blood mononuclear cells (PBMC) phosphorylation

SCI

英语

通讯

Immunology

Immunology

WOS:000777781700001

FRONTIERS MEDIA SA

Web of Science

1.Jinan Univ, Dept Nephrol, Affiliated Hosp 1, Guangzhou, Peoples R China
2.Jinan Univ, Affiliated Hosp 1,Southern Univ Sci & Technol, Guangdong Prov Engn Res Ctr Autoimmune Dis Precis, Clin Med Coll 2,Shenzhen Peoples Hosp, Shenzhen, Peoples R China
3.Heidelberg Univ, Univ Med Ctr Mannheim, Dept Med Nephrol Endocrinol Rheumatol 5, Heidelberg, Germany

Yu, Zongchao,Hong, Xiaoping,Zhang, Xiaoli,et al. Global Proteomic Analyses Reveals Abnormal Immune Regulation in Patients With New Onset Ankylosing Spondylitis[J]. Frontiers in Immunology,2022,13.

Yu, Zongchao.,Hong, Xiaoping.,Zhang, Xiaoli.,Zheng, Fengping.,Liu, Fanna.,...&Dai, Yong.(2022).Global Proteomic Analyses Reveals Abnormal Immune Regulation in Patients With New Onset Ankylosing Spondylitis.Frontiers in Immunology,13.

Yu, Zongchao,et al."Global Proteomic Analyses Reveals Abnormal Immune Regulation in Patients With New Onset Ankylosing Spondylitis".Frontiers in Immunology 13(2022).